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- mouse monoclonal IgG1; 200 µg/ml
- specific for an epitope mapping between amino acids 480-505 at the C-terminus of PPAR© of human origin (identical to corresponding mouse sequence)
- recommended for detection of PPARγ1, PPARγ2 and, to a lesser extent, PPARα and PPARβ of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- blocking peptide, sc-7273 P
- agarose conjugate for IP studies, sc-7273 AC, 500 µg/0.25 ml agarose
- TransCruz reagent for Gel Supershift and ChIP applications, sc-7273 X, 200 µg/0.1 ml
- fluorescein (sc-7273 FITC) and rhodamine (sc-7273 TRITC) conjugates for immunofluorescence, 200 µg/ml
- Alexa Fluor® 405 (sc-7273 AF405), Alexa Fluor® 488 (sc-7273 AF488) and Alexa Fluor® 647 (sc-7273 AF647) conjugates for immunofluorescence; 100 µg/2 ml
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PPARγ Background Information
Peroxisome proliferator-activated receptors (PPARs) are members of the nuclear hormone receptor subfamily of transcription factors. PPARs form heterodimers with retinoid X receptors (RXRs). These heterodimers regulate transcription of genes involved in insulin action, adipocyte differentiation, lipid metabolism and inflammation. PPAR© is implicated in numerous diseases including obesity, diabetes, atherosclerosis and cancer. PPAR© activators include prostanoids, fatty acids, thiazolidinediones and N-(2-benzoylphenyl) tyrosine analogues. A key component in adipocyte differentiation and fat-specific gene expression, PPAR© may modulate macrophage functions such as proinflammatory activities, and stimulate oxidized low-density lipoprotein (x-LDL) uptake. A Pro12Ala polymorphism of the PPAR©2 gene has been reported to reduce transactivation activity in vitro. This substitution may affect the immune response to ox-LDL and be associated with type 2 diabetes. In addition, the Pro12Ala variant of the PPAR©2 gene maybe correlated with abdominal obesity in type 2 diabetes.
| PPARγ (E-8) Product Citations |
See how others have used PPARγ (E-8): sc-7273 antibody and or PPARγ (E-8) antibody conjugates.
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PPARγ (E-8)
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PPARγ (E-8): sc-7273. Immunofluorescence staining of methanol-fixed Jurkat cells (A) and immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue (B) showing nuclear localization.
PPARγ (E-8): sc-7273. Western blot analysis of PPARγ expression in U-937 whole cell lysate (A), wild type human PPARγ (B) and human PPARγ-Pax recombinant fusion protein (C). PPARγ-Pax protein kindly provided by Todd Kroll, Emory University.
PPARγ (E-8): sc-7273. Immunoperoxidase staining of formalin fixed, paraffin-embedded human oral mucosa tissue showing nuclear and cytoplasmic staining of surface epithelial cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
PPARγ (E-8): sc-7273. Western blot analysis of PPARγ expression in non-transfected 293T: sc-117752 (A), human PPARγ transfected 293T: sc-110516 (B) and THP-1 (C) whole cell lysates. PPARγ (E-8): sc-7273. Western blot analysis of PPARγ expression in non-transfected 293T: sc-117752 (A), mouse PPARγ transfected 293T: sc-122729 (B) and U-937 (C) whole cell lysates. PPARγ (E-8): sc-7273. Western blot analysis of human recombinant PPARγ.
PPARγ (E-8): sc-7273. Western blot analysis of PPARγ expression in non-transfected 293T: sc-117752 (A), human PPARγ transfected 293T: sc-159760 (B) and THP-1 (C) whole cell lysates. PPARγ (E-8): sc-7273. Western blot analysis of PPARγ expression in non-transfected 293T: sc-117752 (A), human PPARγ transfected 293T: sc-159760 (B) and THP-1 (C) whole cell lysates. |
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