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Omni-probe (D-8) Antibody: sc-7270 |
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- mouse monoclonal IgG2a; 200 µg/ml
- specific for an epitope mapping between amino acids 8-30 within the (His)6 and polylinker sequence
- recommended for detection of fusion proteins encoded by the prokaryotic pRSET A, B, C and pTrc His A, B, C; baculovirus pBlue Bac His A, B, C and mammalian pEBV His A, B, C expression vectors by WB, IP and ELISA
- blocking peptide, sc-7270 P
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Omni-probe Background Information
Plasmid vectors for the expression of coding regions of eukaryotic genes in E. coli, insect and mammalian hosts are in common usage. Such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part eukaryotic specified proteins (1). For instance, the prokaryotic pRSET A, B, C and pTrc His A, B, C; baculovirus pBlue Bac His A, B, C and mammalian pEBV His A, B, C expression vectors encode fusion proteins containing polyhistidine sequences fused to insertion cDNA sequences allowing for rapid purification on nickel-charged agarose resin (2-4). Omni-probe antibodies directed to specific vector sequences mapping between the polyhistidine tag and cDNA insert sequences provide a convenient and rapid means for identification of expressed fusion proteins in different hosts.
| Omni-probe (D-8) Product Citations |
See how others have used Omni-probe (D-8): sc-7270 antibody and or Omni-probe (D-8) antibody conjugates.
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Omni-probe (D-8)
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Omni-probe (D-8): sc-7270. Western blot analysis of Omni-tag fusion proteins, Syk (257-352): sc-4063 WB (A) and ATF-1(39-271): sc-4006 WB (B). |
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