specific for an epitope mapping between amino acids 625-648 at the C-terminus of Raf-1 of human origin
recommended for detection of Raf-1 of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA; also reactive with additional species, including equine, canine and bovine
fluorescein (sc-7267 FITC) and phycoerythrin (sc-7267 PE) conjugates for intracellular FCM, 100 tests
Raf-1 Background Information Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk)and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-1 is activated in response to activation of a variety of tyrosine kinase receptors as well as in response to pp60v-Src expression. There is accumulating evidence that Ras p21 may play a role in activation of Raf-1 and may play the role of the messenger from membrane tyrosine kinases to Raf-1.
Raf-1 (E-10) Product Citations
See how others have used Raf-1 (E-10): sc-7267 antibody and or Raf-1 (E-10) antibody conjugates.
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Raf-1 (E-10)
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Raf-1 (E-10): sc-7267. Immunoperoxidase staining of formalin- fixed, paraffin-embedded human breast carcinoma tissue (A) and immunofluorescence staining of methanol-fixed NIH/3T3 cells (B) showing cytoplasmic and membrane staining.
Raf-1 (E-10): sc-7267. Western blot analysis of Raf-1 expression in 3611-RF whole cell lysate.
Raf-1 (E-10) PE: sc-7267 PE. Intracellular FCM analysis of fixed and permeabilized NIH/3T3 cells. Black line histogram represents the isotype control, normal mouse IgG1: sc-2866.
Raf-1 (E-10): sc-7267. Western blot analysis of Raf-1 expression in non-transfected: sc-117752 (A) and human Raf-1 transfected: sc-113164 (B) 293T whole cell lysates.
Raf-1 (E-10): sc-7267. Western blot analysis of Raf-1 expression in non-transfected: sc-117752 (A) and human Raf-1 transfected: sc-113164 (B) 293T whole cell lysates.
Raf-1 (E-10): sc-7267. Western blot analysis of Raf-1 expression in non-transfected: sc-117752 (A) and mouse Raf-1 transfected: sc-122942 (B) 293T whole cell lysates.
