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- goat polyclonal IgG, 200 µg/ml
- epitope mapping at the C-terminus of NCoA-3 of human origin
- recommended for detection of NCoA-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including equine, canine, bovine and porcine
- blocking peptide, sc-7216 P
- TransCruz reagent for ChIP application, sc-7216 X, 200 µg/0.1 ml
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NCoA-3 Background Information
Nuclear receptors for steroids, thyroid hormones and retinoic acids are ligand-dependent transcription factors that activate transcription through specific DNA binding sites in their target genes. Several related transcriptional coactivators and corepressors have been described that work in concert with the steroid receptor family to either induce or repress transcription from hormone-responsive elements. This family includes GRIP1 (for GR interacting protein 1), also designated NCoA-2 or TIF-2; SRC-1 (for steroid receptor coactivator-1), also designated NCoA-1; and NCoA-3, also designated RAC3, ACTR, AIB-1 (for amplified in breast cancer) and p/CIP (for p300/CBP/co-integrator protein), which displays elevated expression in estrogen receptor positive ovarian and breast cancers and is required for the transcriptional activation of p300/CBP-dependent transcription factors.
| NCoA-3 (C-20) Product Citations |
See how others have used NCoA-3 (C-20): sc-7216 antibody and or NCoA-3 (C-20) antibody conjugates.
15 total citations Loading citations.
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NCoA-3 (C-20)
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NCoA-3 (C-20): sc-7216. Western blot analysis of NCoA-3 expression in HeLa nuclear extract.
NCoA-3 (C-20): sc-7216. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining.
ChIP analysis of cofactor occupancy dynamics on the ICAM1 promoter in 293 cells in response to IL-1β treatment. Antibodies tested include p300 (C-20): sc- sc-585, p300 (N-15): sc-584, p300 (H-272): sc-8981, NCoA-3 (F-2): sc-5305, NCoA-3 (M-397): sc-9119, NCoA-3 (N-17): sc-7217, NCoA-3 (C-20): sc-7216, SRC-1 (M-341): sc-8995, SRC-1 (C-20): sc-6096, SRC-1 (M-20): sc-6098 and GRIP1 (M-343): sc-8996. Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67. ChIP analysis of cofactor occupancy dynamics on the IL-8 promoter in 293 cells in response to IL-1β treatment. Antibodies tested include NCoA-3 (F-2): sc-5305, NCoA-3 (M-397): sc-9119, NCoA-3 (N-17): sc-7217, NCoA-3 (C-20): sc-7216, SRC-1 (M-341): sc-8995, SRC-1 (C-20): sc-6096, SRC-1 (M-20): sc-6098, GRIP1 (M-343): sc-8996, PCAF (C-16): sc-6300 and PCAF (H-369): sc-8999. Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67.
ChIP analysis of in vivo binding of RORα and its recruitment of coactivators to RORα-responsive promoters in freshly dissected cerebella derived from wild type (+/+) and staggerer (Sg) mice. Control Input (A). Antibodies used included SRC-1 (M-341): sc-8995, SRC-1 (C-20): sc-6096 and SRC-1 (M-20): sc-6098 (B), Grip-1 (M-434): sc-8996 (C), NCoA-3 (F-2): sc-5305, NCoA-3 (M-397): sc-9119, NCoA-3 (N-17): sc-7217 and NCoA-3 (C-20): sc-7216 (D). Data kindly provided by M.G. Rosenfeld and reproduced wtih permission from Gold et al., Neuron 2003, 40: 1119-1131. NCoA-3 siRNA (h): sc-29636. Western blot analysis of NCoA-3 expression in non-transfected control (A) and NCoA-3 siRNA transfected (B) HeLa cells. Blot probed with NCoA-3 (C-20): sc-7216. α-actinin (H-2): sc-17829 used as specificity and loading control. NCoA-3 (C-20): sc-7216. Western blot analysis of NCoA-3 expression in rat testis tissue extract.
NCoA-3 (C-20): sc-7216. Western blot analysis of NCoA-3 expression in K-562 whole cell lysate.
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