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caspase-8 Antibody (4i30): sc-70502

 |  Datasheet

(Based on data analysis)

  • caspase-8 Antibody (4i30) is a mouse monoclonal IgG1 provided at 200 µg/ml
  • raised against recombinant full length human caspase-8 protein
  • recommended for detection of caspase-8 of human origin by WB, IP, IF and IHC(P)
 

See additional Caspase Antibodies including caspase-14, caspase-12, caspase-11, caspase-10, caspase-9, caspase-8, caspase-7, caspase-6, caspase-5, caspase-4, caspase-3, caspase-2, caspase-1, Caspase and caspase-16.

Also see caspase-8 Inhibitors, caspase-8 Activators or caspase-8 Substrates for functional analysis of cellular responses to caspase-8.


Ordering InformationGene Info
Recommended Support Products:
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WB   IP   IF   IHC(P)   siRNA  
Set Currency

 Ordering Information
Product NameCatalog #UnitPriceQtyAdd 
caspase-8 Antibody (4i30) sc-70502 200 µg/ml $279
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
caspase-8 siRNA (h) sc-29930 10 µM $258
caspase-8 (h)-PR sc-29930-PR 10 µM, 20 µl $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
caspase-8 shRNA Plasmid (h) sc-29930-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
caspase-8 shRNA (h) Lentiviral Particles sc-29930-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
Jurkat Whole Cell Lysate sc-2204 500 µg/200 µl $104
HL-60 Whole Cell Lysate sc-2209 500 µg/200 µl $104
SW480 Cell Lysate sc-2219 500 µg/200 µl $104
MOLT-4 Cell Lysate sc-2233 500 µg/200 µl $104
CCRF-CEM Cell Lysate sc-2225 500 µg/200 µl $104
THP-1 Cell Lysate sc-2238 500 µg/200 µl $104
K-562 Whole Cell Lysate sc-2203 500 µg/200 µl $104
HeLa Whole Cell Lysate sc-2200 500 µg/200 µl $104
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human CASP8 841 2q33.1 NM_001228, NM_033355, NM_033356, NM_033358 Q14790
 607271
 

Initiator caspases, which include caspase-8, activate effector caspases by cleaving inactive forms of effector caspases. In the activation cascade responsible for apoptosis induced by TNFRSF1A and mediated by TNFRSF6/FAS, caspase-8 is the most upstream protease. Caspase-8 binds to adaptor molecule FADD, forming an aggregate referred to as death-inducing signaling complex (DISC), which activates caspase-8. The actived protein is released from the complex and further activates downstream apoptotic proteases. Caspase-8, which is a heterodimer consisting of two subunits (p18 and p10), is widely expressed, but is detected at highest levels in peripheral blood leukocytes (PBLs), thymus, liver and spleen. Defects in CASP8, the gene encoding for caspase-8, may cause CASP8D (caspase-8 deficiency disorder), which is characterized by splenomegaly and CD95-induced apoptosis of PBLs, may lead to immunodeficiency due to defects in T lymphocyte, NK cell and B lymphocyte activation.
caspase-8 Antibody (4i30) Data
Click on image to enlarge
sc-70502
caspase-8 (4i30): sc-70502. Western blot analysis of caspase-8 precursor expression in MOLT-4 whole cell lysate.
sc-70502
caspase-8 (4i30): sc-70502. Western blot analysis of caspase-8 precursor expression in Jurkat whole cell lysate.
sc-70502
caspase-8 (4i30): sc-70502. Western blot analysis of caspase-8 cleavage in untreated (A) and Staurosporine (sc-3510) treated (B) Jurkat whole cell lysates. Note caspase-8 cleavage product expression in lane B.
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