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p-Tyr Antibody (PY99): sc-7020

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  • p-Tyr Antibody (PY99) is a mouse monoclonal IgG2b provided at 200 µg/ml
  • specific detection of phosphotyrosine-containing proteins by WB, IP, IF and IHC(P); non cross-reactive with phosphoserine or phosphothreonine
  • agarose conjugate for IP studies, p-Tyr (PY99) AC: sc-7020 AC, 500 µg/0.25 ml agarose
  • agarose (sc-7020 AC), 500 µg/0.25 ml agarose in 1 ml, for IP; HRP (sc-7020 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; phycoerythrin (sc-7020 PE), fluorescein (sc-7020 FITC), Alexa Fluor® 488 (sc-7020 AF488) or Alexa Fluor® 647 (sc-7020 AF647), 200 µg/ml, for IF, IHC(P) and FCM
  • biotin (sc-7020 B), 200 µg/ml, for WB, IHC(P) and ELISA;Alexa Fluor® 405 (sc-7020 AF405), 100 µg/2 ml, for IF, IHC(P) and FCM.
  • See 600 citations for p-Tyr Antibody (PY99)
 

See additional Antibodies to Phosphorylated Amino Acids including Antibodies to Phosphorylated Amino Acids, p-Ser, p-Thr and p-Tyr.

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 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
p-Tyr Antibody (PY99) sc-7020 200 µg/ml $279
p-Tyr Antibody (PY99) AC sc-7020 AC 500 µg/ml, 25% ag $366
p-Tyr Antibody (PY99) Alexa Fluor® 405 sc-7020 AF405 100 µg/2 ml $314
p-Tyr Antibody (PY99) Alexa Fluor® 488 sc-7020 AF488 200 µg/ml $314
p-Tyr Antibody (PY99) Alexa Fluor® 647 sc-7020 AF647 100 µg/2 ml $314
p-Tyr Antibody (PY99) AP sc-7020 AP 200 µg/ml $282
p-Tyr Antibody (PY99) B sc-7020 B 200 µg/ml $282
p-Tyr Antibody (PY99) FITC sc-7020 FITC 200 µg/ml $292
p-Tyr Antibody (PY99) HRP sc-7020 HRP 200 µg/ml $279
p-Tyr Antibody (PY99) PE sc-7020 PE 200 µg/ml $303
 WB Positive Control Cell Lysate (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
A-431 + EGF Whole Cell Lysate sc-2202 500 µg/200 µl $104


The critical involvement of protein tyrosine kinases in signal transduction pathways is well established. These kinases can be divided into two major groups, including the receptor tyrosine kinases and the non-receptor type kinases, of which the Src kinases are the prototypical members. Src kinases are generally associated with the internal portion of the plasma membrane and may function as signal transducers in association with surface receptors that lack an intracellular catalytic domain. The second major group of tyrosine kinases are the receptor tyrosine kinases. More than fifty members of this group of receptors, belonging to fourteen families, have been identified to date. Ligand-induced tyrosine phosphorylation of such receptors induces receptor dimerization and subsequent autophosphorylation of specific individual phosphotyrosine residues located within their cytoplasmic domains, which serve as binding sites that interact with specific cytoplasmic molecules. Monoclonal antibodies to phosphotyrosine are valuable for the characterization and purification of proteins containing phosphotyrosyl residues, and are used extensively for these purposes.

p-Tyr Antibody (PY99) Product Reviews

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p-Tyr Antibody (PY99) Product Citations
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p-Tyr Antibody (PY99) Data
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p-Tyr (PY99): sc-7020. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing membrane localization of tyrosine phosphorylated proteins.
p-Tyr (PY99): sc-7020. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.
Western blot analysis of p-Tyr phosphorylation in untreated (A,C) and EGF treated (B,D) A-431 whole cell lysates. Antibodies tested include p-Tyr (PY99): sc-7020 (A,B) and p-Tyr (PY20): sc-508 (C,D).
p-Tyr (PY99): sc-7020. Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human tonsil at low (A) and high (B) magnification showing membrane localization of tyrosine phosphorylated proteins.
p-Tyr (PY99): sc-7020. Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human skin tissue showing membrane and cytoplasmic localization of tyrosine phosphorylated proteins.
p-Tyr (PY99): sc-7020. Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human urinary bladder tissue showing membrane and cytoplasmic localization of tyrosine phosphorylated proteins.
p-Tyr (PY99) Alexa Fluor® 488: sc-7020 AF488. Direct immunofluorescence staining of formalin-fixed HeLa cells showing membrane and focal adhesion sites (green) and nuclear DAPI counterstain (blue) localization.
p-Tyr (PY99) Alexa Fluor 488: sc-7020 AF488. Direct immunofluorescence staining of formalin-fixed HeLa cells showing membrane and focal adhesion sites localization.
p-Tyr (PY99) HRP: sc-7020 HRP. Direct western blot analysis of p-Tyr expression in A-431 whole cell lysates prepared from non-transfected cells (A) and EGF transfected cells (B).
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