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p-Tyr Antibody (PY99): sc-7020

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  • p-Tyr Antibody (PY99) is a mouse monoclonal IgG2b provided at 200 µg/ml
  • specific detection of phosphotyrosine-containing proteins by WB, IP, IF and IHC(P); non cross-reactive with phosphoserine or phosphothreonine
  • agarose conjugate for IP studies, p-Tyr (PY99) AC: sc-7020 AC, 500 µg/0.25 ml agarose
  • HRP conjugate; p-Tyr (PY99) HRP: sc-7020 HRP, 200 µg/ml
  • fluorescein (p-Tyr (PY99) FITC: sc-7020 FITC) and phycoerythrin (p-Tyr (PY99) PE: sc-7020 PE) conjugates for intracellular FCM, 100 tests
  • AP conjugate; p-Tyr (PY99) AP: sc-7020 AP, 200 µg/ml
  • biotin conjugate, p-Tyr (PY99) B: sc-7020 B, 200 µg/ml
  • Alexa Fluor® 405 (sc-7020 AF405), Alexa Fluor® 488 (sc-7020 AF488) and Alexa Fluor® 647 (sc-7020 AF647) conjugates for FCM or immunofluorescence; 100 µg/2 ml
  • See 563 citations for p-Tyr Antibody (PY99)

See additional Antibodies to Phosphorylated Amino Acids including Antibodies to Phosphorylated Amino Acids, p-Ser, p-Thr and p-Tyr.

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 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
p-Tyr Antibody (PY99) sc-7020 200 µg/ml $279
p-Tyr Antibody (PY99) AC sc-7020 AC 500 µg/ml, 25% ag $366
p-Tyr Antibody (PY99) Alexa Fluor® 405 sc-7020 AF405 100 µg/2 ml $314
p-Tyr Antibody (PY99) Alexa Fluor® 488 sc-7020 AF488 100 µg/2 ml $314
p-Tyr Antibody (PY99) Alexa Fluor® 647 sc-7020 AF647 100 µg/2 ml $314
p-Tyr Antibody (PY99) AP sc-7020 AP 200 µg/ml $282
p-Tyr Antibody (PY99) B sc-7020 B 200 µg/ml $282
p-Tyr Antibody (PY99) FITC sc-7020 FITC 100 tests in 2 ml $292
p-Tyr Antibody (PY99) HRP sc-7020 HRP 200 µg/ml $279
p-Tyr Antibody (PY99) PE sc-7020 PE 100 tests in 2 ml $303
 WB Positive Control Cell Lysate (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
A-431 + EGF Whole Cell Lysate sc-2202 500 µg/200 µl $104

The critical involvement of protein tyrosine kinases in signal transduction pathways is well established. These kinases can be divided into two major groups, including the receptor tyrosine kinases and the non-receptor type kinases, of which the Src kinases are the prototypical members. Src kinases are generally associated with the internal portion of the plasma membrane and may function as signal transducers in association with surface receptors that lack an intracellular catalytic domain. The second major group of tyrosine kinases are the receptor tyrosine kinases. More than fifty members of this group of receptors, belonging to fourteen families, have been identified to date. Ligand-induced tyrosine phosphorylation of such receptors induces receptor dimerization and subsequent autophosphorylation of specific individual phosphotyrosine residues located within their cytoplasmic domains, which serve as binding sites that interact with specific cytoplasmic molecules. Monoclonal antibodies to phosphotyrosine are valuable for the characterization and purification of proteins containing phosphotyrosyl residues, and are used extensively for these purposes.

p-Tyr Antibody (PY99) Product Reviews

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p-Tyr Antibody (PY99) Product Citations
See how others have used p-Tyr Antibody (PY99) and or p-Tyr Antibody (PY99) conjugates.

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p-Tyr Antibody (PY99) Data
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p-Tyr (PY99): sc-7020. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing membrane localization of tyrosine phosphorylated proteins.
p-Tyr (PY99): sc-7020. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.
Western blot analysis of p-Tyr phosphorylation in untreated (A,C) and EGF treated (B,D) A-431 whole cell lysates. Antibodies tested include p-Tyr (PY99): sc-7020 (A,B) and p-Tyr (PY20): sc-508 (C,D).
p-Tyr (PY99): sc-7020. Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human tonsil at low (A) and high (B) magnification showing membrane localization of tyrosine phosphorylated proteins.