 |
- mouse monoclonal IgG1; 200 µg/ml
- raised against amino acids 108-300 of E2F-4 of human origin
- recommended for detection of E2F-4 of human origin by WB, IP, IF and IHC(P)
- TransCruz reagent for Gel Supershift and ChIP applications, sc-6851 X, 200 µg/0.1 ml
- agarose conjugate for IP studies, sc-6851 AC, 500 µg/0.25 ml agarose
- HRP conjugate, sc-6851 HRP, 200 µg/ml
|
|
| |
Ordering InformationProduct Citations
Recommended Support Products:
(click button of application of choice)
| |
| Species |
Gene Name |
Gene ID |
Chromosome Location |
Isoform (mRNA) Accession # |
Protein Accession # |
OMIM™ Number |
| Human |
E2F4 |
1874 |
16q22.1 |
NM_001950 |
Q16254
|
600659 |
| |
E2F-4 Background Information
The human retinoblastoma gene product appears to play an important role in the negative regulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus-encoded proteins. Of all the Rb associations described to date, the identification of a complex between Rb and the transcription factor E2F most directly implicates Rb in regulation of cell proliferation. E2F was originally identified through its role in transcriptional activation of the adenovirus E2 promoter. Sequences homologous to the E2F binding site have been found upstream of a number of genes that encode proteins with putative functions in the G1 and S phases of the cell cycle. E2F-1 is a member of a broader family of transcription regulators including E2F-2, E2F-3, E2F-4, E2F-5, E2F-6 and E2F-7 each of which forms heterodimers with a second protein, DP-1, forming an “active” E2F transcriptional regulatory complex.
| E2F-4 (D-3) Product Citations |
See how others have used E2F-4 (D-3): sc-6851 antibody and or E2F-4 (D-3) antibody conjugates.
3 total citations Loading citations.
|
| |
|
|
|
E2F-4 (D-3)
Click on image to enlarge
|
|
E2F-4 (D-3): sc-6851. Western blot analysis of human recombinant E2F-4 fusion protein (A). An unrelated fusion protein with the same tag (B) and the tag alone (C) were included as controls.
E2F-4 (D-3): sc-6851. Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human colon tissue. Note nuclear staining of epithelia.
ChIP analysis of c-Myc promoter occupancy in response to lithium stimulation in serum synchronized C2C12 cells. Antibodies tested include HDAC1 (H-15): sc-7872, HDAC1 (C-19): sc-6298 and HDAC1 (H-11): sc-8410 (A), β-catenin (H-120): sc-7199, β-catenin (C-18): sc-1496 and β-catenin (E-5): sc-7963 (B), E2F-4 (A-20): sc-1082, E2F-4 (D-3): sc-6851, E2F-4 (RK-13): sc-511 and E2F-4 (C-20): sc-866 (C), and p130 (C-20): sc-317, p130 (211.6): sc-9963 and p130 (H-125): sc-20678 (D). Data kindly provided by M.G. Rosenfeld and reproduced with permission from Proc. Natl. Acad. Sci. 100: 3245-3250. Copyright 2003 National Academy of Sciences, USA. E2F-4 (D-3): sc-6851. Western blot analysis of E2F-4 expression in MEG-01 nuclear extract.
E2F-4 (D-3): sc-6851. Western blot analysis of E2F-4 expression in non-transfected: sc-117752 (A) and mouse E2F-4 transfected: sc-119883 (B) 293T whole cell lysates. |
|
Download
|
|
