epitope mapping within an internal region of MLTK of human origin
recommended for detection of MLTK of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine, canine, bovine, porcine and avian
MLTK Background Information MLTK (mitogen-activated protein kinase kinase kinase MLT, leucine zipper- and sterile a motif-containing kinase, HCCS-4) is an 800 amino acid protein encoded by the human gene ZAK. MLTK belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family, MAP kinase kinase kinase subfamily and contains one protein kinase domain and one SAM (sterile a motif) domain. MLTK is a cytoplasmic protein found as a homodimer. It will translocate to the nucleus upon ultraviolet B irradiation. It is a stress-activated component of a protein kinase signal transduction cascade and helps regulates the JNK and p38 pathways. MLTK also has a role in the regulation of S and G2 cell cycle checkpoint by direct phosphorylation of Chk2. Isoform 1 causes cell shrinkage and disruption of Actin stress fibers and may have a role in neoplastic cell transformation and cancer development. Isoform 1 also phosphorylates Histone H3 at Ser 28.
MLTK (D-18)
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MLTK (D-18): sc-67682. Western blot analysis of MLTK expression in NIH/3T3 whole cell lysate.
