PRDM1/Blimp-1 Antibody (3H2E8): sc-66015

Blimp-1 Antibody (3H2E8) is a mouse monoclonal IgG1 kappa light chain antibody that detects Blimp-1 in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF) applications. Anti-Blimp-1 antibody (3H2E8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Blimp-1, also known as B lymphocyte-induced maturation protein or PRDI-BF1, plays a crucial role in plasma cell development and differentiation, representing the terminally differentiated form of B-cells responsible for antibody production. This transcriptional repressor localizes in the nucleus, acting as a master regulator of terminal B-cell differentiation. Blimp-1 triggers the expression of key proteins such as syndecan-1 and J chain, while repressing IFN-β gene transcription and recruiting histone deacetylase to DNA, inhibiting c-myc expression. Blimp-1 expression occurs during late B-cell differentiation stages, particularly in immunoglobulin-secreting plasma cells and long-lived bone marrow plasma cells. Expression serves as a critical checkpoint determining whether fully activated B cells proceed to the plasma cell stage, eliminating immature and partially activated cells. This regulatory function highlights Blimp-1′s importance in maintaining immune response balance and functionality.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PRDM1/Blimp-1 Antibody (3H2E8) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-PRDM1/Blimp-1 Antibody (3H2E8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PRDM1/Blimp-1 Antibody (3H2E8) Specifications

Alternate Product Name: PRDM1

Product Clone Name: 3H2E8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 199-409

Epitope Target: Blimp-1

Epitope Species: mouse

Product Reactivity/Detection: Blimp-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 12 citations in various scientific publications

Available Conjugates/Forms for PRDM1/Blimp-1 Antibody (3H2E8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred PRDM1/Blimp-1 antibody is PRDM1/Blimp-1 Antibody (6D3): sc-47732.