γ-Actin Antibody (1-17): sc-65638

γ-Actin Antibody (1-17) is a mouse monoclonal IgG1 kappa light chain antibody that detects gamma-Actin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-gamma-Actin antibody (1-17) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Actin is a vital protein that plays a crucial role in various cellular functions, including maintaining cell shape, enabling motility, and facilitating intracellular transport. Gamma-Actin is predominantly located in the cytoplasm, where gamma-Actin forms dynamic structures known as filaments that are essential for cellular integrity and movement. The ability of gamma-Actin to polymerize and depolymerize allows cells to adapt their shape and respond to environmental cues, which is particularly important in processes such as wound healing and immune responses. In mammalian tissues, gamma-Actin is one of the three main isoforms, alongside α-Actin and β-Actin, with specific roles in non-muscle cells. The regulation of gamma-Actin dynamics is influenced by various proteins, including members of the small GTPase family, which orchestrate the assembly and disassembly of actin filaments, thereby impacting cellular behavior and function.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

γ-Actin Antibody (1-17) References:

1. Cytoplasmic gamma-actin contributes to a compensatory remodeling response in dystrophin-deficient muscle.  |  Hanft, LM., et al. 2006. Proc Natl Acad Sci U S A. 103: 5385-90. PMID: 16565216
2. γ-Actin regulates cell migration and modulates the ROCK signaling pathway.  |  Shum, MS., et al. 2011. FASEB J. 25: 4423-33. PMID: 21908715
3. Ubiquitin ligase TRIM3 controls hippocampal plasticity and learning by regulating synaptic γ-actin levels.  |  Schreiber, J., et al. 2015. J Cell Biol. 211: 569-86. PMID: 26527743
4. Gene deletion of γ-actin impairs insulin-stimulated skeletal muscle glucose uptake in growing mice but not in mature adult mice.  |  Knudsen, JR., et al. 2022. Physiol Rep. 10: e15183. PMID: 35224890
5. A feedback circuitry involving γ-actin, β-actin and nonmuscle myosin-2 A controls tight junction and apical cortex mechanics.  |  Maupérin, M., et al. 2025. Nat Commun. 16: 2514. PMID: 40082413
6. Role of microtubule-associated proteins in the control of microtubule assembly.  |  Maccioni, RB. and Cambiazo, V. 1995. Physiol Rev. 75: 835-64. PMID: 7480164
7. Rho, rac, and cdc42 GTPases regulate the assembly of multimolecular focal complexes associated with actin stress fibers, lamellipodia, and filopodia.  |  Nobes, CD. and Hall, A. 1995. Cell. 81: 53-62. PMID: 7536630
8. A discourse on modeling F-actin.  |  Schutt, CE., et al. 1995. J Struct Biol. 115: 186-98. PMID: 7577239
9. Actin cytoskeleton. Setting the pace of cell movement.  |  Barkalow, K. and Hartwig, JH. 1995. Curr Biol. 5: 1000-2. PMID: 8542272
10. The origins and evolution of eukaryotic proteins.  |  Doolittle, RF. 1995. Philos Trans R Soc Lond B Biol Sci. 349: 235-40. PMID: 8577833
11. Elastic fibres are an essential component of human placental stem villous stroma and an integrated part of the perivascular contractile sheath.  |  Graf, R., et al. 1996. Cell Tissue Res. 283: 133-41. PMID: 8581953
12. Actin bundles in human hair follicles as revealed by confocal laser microscopy.  |  Furumura, M. and Ishikawa, H. 1996. Cell Tissue Res. 283: 425-34. PMID: 8593672

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-γ-Actin Antibody (1-17) has 23 citations in a variety of scientific publications.

Primary antibodies like Anti-γ-Actin Antibody (1-17) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

γ-Actin Antibody (1-17) Specifications

Alternate Product Name: γ-Actin

Product Clone Name: 1-17

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: γ-Actin

Epitope Species: bovine

Product Reactivity/Detection: γ Actin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 23 citations in various scientific publications

Available Conjugates/Forms for γ-Actin Antibody (1-17): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin