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NOS2 Antibody (N-20): sc-651

 |  Datasheet

(Based on data analysis)

  • NOS2 Antibody (N-20) is a rabbit polyclonal IgG provided at 200 µg/ml
  • epitope mapping near the N-terminus of NOS2 of human origin
  • recommended for detection of NOS2 (iNOS) of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA
  • blocking peptide, sc-651 P
  • phycoerythrin (sc-651 PE), PerCP (sc-651 PerCP) and PerCP-Cy5.5 (sc-651 PCPC5) conjugates for intracellular FCM, sc-651 PE, 100 tests
  • fluorescein (sc-651 FITC) and rhodamine (sc-651 TRITC) conjugates for immunofluorescence, 200 µg/ml
  • See 91 citations for NOS2 Antibody (N-20)
 

See additional NOS Antibodies including NOS, NOS1, pan NOS, NOS2, NOS2B and NOS3.

Also see NOS2 Inhibitors for functional analysis of cellular responses to NOS2.


Ordering InformationProduct CitationsGene Info
Recommended Support Products:
(click button of application of choice)
WB   IP   IF   IHC(P)   FCM   siRNA  
Set Currency

 Ordering Information
Product NameCatalog #UnitPriceQtyAdd 
NOS2 Antibody (N-20) sc-651 200 µg/ml $279
NOS2 Antibody (N-20) FITC sc-651 FITC 200 µg/ml $282
NOS2 (N-20) P sc-651 P
(peptide)
 100 µg/0.5 ml $61
NOS2 Antibody (N-20) PCPC5 sc-651 PCPC5 100 tests in 2 ml $310
NOS2 Antibody (N-20) PE sc-651 PE 100 tests in 2 ml $303
NOS2 Antibody (N-20) PerCP sc-651 PerCP 100 tests in 2 ml $310
NOS2 Antibody (N-20) TRITC sc-651 TRITC 200 µg/ml $282
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
NOS2 siRNA (h) sc-29417 10 µM $258
NOS2 siRNA (m) sc-36092 10 µM $258
NOS2 (h)-PR sc-29417-PR 10 µM, 20 µl $23
NOS2 (m)-PR sc-36092-PR 10 µM, 20 µl $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
NOS2 shRNA Plasmid (h) sc-29417-SH 20 µg $520
NOS2 shRNA Plasmid (m) sc-36092-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
NOS2 shRNA (h) Lentiviral Particles sc-29417-V 200 µl $625
NOS2 shRNA (m) Lentiviral Particles sc-36092-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
RAW 264.7 + LPS/IFN-γ Cell Lysate sc-24767 500 µg/200 µl $104
A-431 Whole Cell Lysate sc-2201 500 µg/200 µl $104
BC3H1 Cell Lysate sc-2299 500 µg/200 µl $104
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human NOS2A 4843 17q11.1 NM_000625, NM_153292 P35228
 611162
Mouse Nos2 18126 11 B5 P29477
 N/A
 

Nitric oxide (NO) has a broad range of biological activities and has been implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOSs), the enzymes responsible for synthesis of NO, contain an N-terminal oxygenase domain and a C-terminal reductase domain. NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells. The inducible form of NOS, NOS2 (also designated iNOS), is Ca2+-independent and is expressed in a broad range of cell types.

NOS2 Antibody (N-20) Product Citations
See how others have used NOS2 Antibody (N-20) and or NOS2 Antibody (N-20) conjugates.


91 total citations
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NOS2 Antibody (N-20) Data
Click on image to enlarge
sc-651
NOS2 (N-20) PE: sc-651 PE. Intracellular FCM analysis of fixed and permeabilized untreated (green line histogram) and LPS/PMA-treated (solid orange histogram) RAW 264.7 cells. Dotted pink histogram represents the isotype control, normal rabbit IgG: sc-3871.
sc-651
NOS2 (N-20): sc-651. Immunofluorescence staining of methanol-fixed RAW 264.7 cells stimulated with LPS/IFN-γ showing cytoplasmic staining.
sc-651
NOS2 (N-20): sc-651. Western blot analysis of NOS2 expression in uninduced (A) and LPS/IFN-γ stimulated (B) RAW 264.7 whole cell lysate.
sc-651
NOS2 (N-20): sc-651. Immunoperoxidase staining of formalin fixed, paraffin-embedded human skeletal muscle tissue showing cytoplasmic staining of myocytes.
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