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- rabbit polyclonal IgG, 200 µg/ml; also available as goat IgG, 200 µg/ml, sc-645-G
- epitope mapping at the C-terminus of Sp4 of human origin
- recommended for detection of Sp4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including equine, canine, bovine, porcine and avian
- blocking peptide, sc-645 P
- agarose conjugate for IP studies, sc-645 AC, 500 µg/0.25 ml agarose
- TransCruz reagent for Gel Supershift and ChIP applications, sc-645 X, 200 µg/0.1 ml
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Ordering InformationProduct Citations
Recommended Support Products:
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| Species |
Gene Name |
Gene ID |
Chromosome Location |
Isoform (mRNA) Accession # |
Protein Accession # |
OMIM™ Number |
| Human |
SP4 |
6671 |
7p15.3 |
NM_003112 |
Q02446
|
600540 |
| Mouse |
Sp4 |
20688 |
12 F2 |
|
Q62445
|
N/A |
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Sp4 Background Information
The Sp transcription factor family includes Sp1, Sp2, Sp3 (SPR-2) and Sp4 (SPR-1). Sp transcription factors share similar structures but do not share similar functions. All four proteins contain a highly conserved DNA-binding domain composed of three zinc fingers at the C-terminus. Sp family members bind the consensus sequence GGGGCGGGGC and other closely related sequences which are known as GC boxes. Sp1, Sp3 and Sp4 share a high affinity for GC boxes while Sp2 does not. Sp2 only weakly binds to GT boxes. Sp1, Sp2 and Sp3 are ubiquitously expressed, while Sp4 is abundantly expressed in brain with limited expression in other tissues. Sp1 and Sp3, but not Sp2 or Sp4, interact with E2, a regulatory element for the ∫4 subunit of neuronal nicotinic acetylcholine receptors. Sp3 is the only Sp member to inhibit Sp1 and Sp4 mediated transcription.
| Sp4 (V-20) Product Citations |
See how others have used Sp4 (V-20): sc-645 antibody and or Sp4 (V-20) antibody conjugates.
35 total citations Loading citations.
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Sp4 (V-20)
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Sp4 (V-20): sc-645. Western blot analysis of Sp4 expression in NIH/3T3 (A,B) and KNRK (C,D) nuclear extracts.
Sp4 (V-20): sc-645. Immunofluorescence staining of methanol-fixed KNRK cells showing nuclear staining.
Sp4 siRNA (h): sc-36545. Western blot analysis of Sp4 expression in non-transfected control (A) and Sp4 siRNA transfected (B) HeLa cells. Blot probed with Sp4 (V-20): sc-645. α-actinin (H-2): sc-17829 used as specificity and loading control. Sp4 (V-20): sc-645. ChIP analysis of Sp4 recruitment to genomic amplicons. Four (1-4) different human genomic Sp-1 and two control amplicons (5,6) were analyzed by quantitative PCR (primer sequences available in on-line supplemental data). Data generated in collaboration with Drs. N. Trinklein and R. Myers, Stanford University (ENCODE Project).
Sp4 (V-20): sc-645. Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing nuclear staining of cells in granular and molecular layers and cytoplasmic staining of Purkinje cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
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