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- rabbit polyclonal IgG, 200 µg/ml; also available as goat IgG, 200 µg/ml, sc-644-G
- epitope mapping at the C-terminus of Sp3 of human origin
- recommended for detection of Sp3 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine, canine, bovine, porcine and avian
- blocking peptide, sc-644 P
- fluorescein (sc-644 FITC) and rhodamine (sc-644 TRITC) conjugates for immunofluorescence, 200 µg/ml
- TransCruz reagent for Gel Supershift and ChIP applications, sc-644 X, 200 µg/0.1 ml
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Sp3 Background Information
The Sp transcription factor family includes Sp1, Sp2, Sp3 (SPR-2) and Sp4 (SPR-1). Sp transcription factors share similar structures but do not share simi-lar functions. All four proteins contain a highly conserved DNA-binding domain composed of three zinc fingers at the C-terminus. Sp family members bind the consensus sequence GGGGCGGGGC and other closely related sequences which are known as GC boxes. Sp1, Sp3 and Sp4 share a high affinity for GC boxes while Sp2 does not. Sp2 only weakly binds to GT boxes. Sp1, Sp2 and Sp3 are ubiquitously expressed, while Sp4 is abundantly expressed in brain with limited expression in other tissues. Sp1 and Sp3, but not Sp2 or Sp4, interact with E2, a regulatory element for the ∫4 subunit of neuronal nicotinic acetylcholine receptors. Sp3 is the only Sp member to inhibit Sp1 and Sp4 mediated transcription. Multiple isoforms of Sp3 exist due to alternative splicing events.
| Sp3 (D-20) Product Citations |
See how others have used Sp3 (D-20): sc-644 antibody and or Sp3 (D-20) antibody conjugates.
144 total citations Loading citations.
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Sp3 (D-20)
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Sp3 (D-20)-G: sc-644-G. Western blot analysis of Sp3 isoform expression in Jurkat whole cell lysate (A) and KNRK nuclear extract (B). Antibodies tested include Sp3 (D-20)-G: sc-644-G (A) and Sp3 (D-20): sc-644 (B). Sp3 (D-20): sc-644. Nuclear immunofluorescence staining of methanol-fixed HeLa cells.
Sp3 siRNA (h): sc-29490. Western blot analysis of Sp3 expression in non-transfected control (A) and Sp3 siRNA transfected (B) HeLa cells. Blot probed with Sp3 (D-20): sc-644. α-actinin (H-2): sc-17829 used as specificity and loading control. Sp3 (D-20): sc-644. ChIP analysis of Sp3 recruitment to genomic amplicons. Four (1-4) different human genomic Sp-1 and two control amplicons (5,6) were analyzed by quantitative PCR (primer sequences available in on-line supplemental data). Data generated in collaboration with Drs. N. Trinklein and R. Myers, Stanford University (ENCODE Project).
Sp3 (D-20): sc-644. Immunofluorescence staining of normal mouse liver frozen section showing nuclear staining.
Sp3 (D-20): sc-644. Western blot analysis of Sp3 expression in non-transfected: sc-117752 (A) and mouse Sp3 transfected: sc-127572 (B) 293T whole cell lysates and HeLa nuclear extract (C). Sp3 (D-20)-G: sc-644-G. Western blot analysis of Sp3 expression in non-transfected: sc-117752 (A) and mouse Sp3 transfected: sc-127572 (B) 293T whole cell lysates. |
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