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- rabbit polyclonal IgG, 200 µg/ml
- epitope mapping at the C-terminus of E2F-2 of human origin
- recommended for detection of E2F-2 of mouse and human origin by WB, IP, IF and ELISA; partially cross-reactive with E2F-3 and E2F-1; also reactive with additional species, including canine and bovine
- blocking peptide, sc-633 P
- TransCruz reagent for Gel Supershift and ChIP applications, sc-633 X, 200 µg/0.1 ml
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Ordering InformationProduct Citations
Recommended Support Products:
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| Species |
Gene Name |
Gene ID |
Chromosome Location |
Isoform (mRNA) Accession # |
Protein Accession # |
OMIM™ Number |
| Human |
E2F2 |
1870 |
1p36.12 |
NM_004091 |
Q14209
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600426 |
| Mouse |
E2f2 |
242705 |
4 D3 |
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P56931
|
N/A |
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E2F-2 Background Information
The human retinoblastoma gene product appears to play an important role in the negative regulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus-encoded proteins. Of all the Rb associations described to date, the identification of a complex between Rb and the transcription factor E2F most directly implicates Rb in regulation of cell proliferation. E2F was originally identified through its role in transcriptional activation of the adenovirus E2 promoter. Sequences homologous to the E2F binding site have been found upstream of a number of genes that encode proteins with putative functions in the G1 and S phases of the cell cycle. E2F-1 is a member of a broader family of transcription regulators including E2F-2, E2F-3, E2F-4, E2F-5, E2F-6 and E2F-7 each of which forms heterodimers with a second protein, DP-1, forming an “active” E2F transcriptional regulatory complex.
| E2F-2 (C-20) Product Citations |
See how others have used E2F-2 (C-20): sc-633 antibody and or E2F-2 (C-20) antibody conjugates.
62 total citations Loading citations.
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E2F-2 (C-20)
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Western blot analysis of E2F-2 expression in phorbol ester-induced A-431 nuclear extracts (A,B). Antibodies tested include E2F-2 (L-20): sc-632 (A) and E2F-2 (C-20): sc-633 (B). ChIP analysis of E2F transcription factor binding to the c-Myc promoter in NBS cells. Antibodies tested include E2F-1 (C-20): sc-193 (A), E2F-2 (C-20): sc-633 (B), E2F-3 (C-18): sc-878, E2F-3 (N-20): sc-879 (C) and E2F-4 (C-20): sc-866 (D). Data kindly provided by Dr. J. H. J. Petrini and reproduced with permission from Master, et al. 2001. Mol. Cell. Biol. 21: 6006-6016. ChIP analysis of E2F transcription factor binding to the oriP (I.) and c-Myc (II.) promoters in Raji cells as a function of the cell cycle. Antibodies tested include E2F-1 (C-20): sc-193 (A), E2F-2 (C-20): sc-633 (B), E2F-3 (C-18): sc-878, E2F-3 (N-20): sc-879 (C) and E2F-4 (C-20): sc-866 (D). Data kindly provided by Dr. J. H. J. Petrini and reproduced from Master, et al., 2001. Mol. Cell. Biol. 21: 6006-6016. E2F-2 (C-20): sc-633. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
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