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Vimentin Antibody (V9): sc-6260

 |  Datasheet

(Based on data analysis)

  • Vimentin Antibody (V9) is a mouse monoclonal IgG1 provided at 200 µg/ml
  • raised against purified Vimentin from porcine eye lens
  • recommended for detection of Vimentin of r, h, avian and porcine origin (non cross-reactive with mouse) by WB, IP, IF, IHC(P) and FCM
  • fluorescein (sc-6260 FITC) and rhodamine (sc-6260 TRITC) conjugates for immunofluorescence and FCM, 200 µg/ml
  • phycoerythrin (sc-6260 PE), PerCP (sc-6260 PerCP) and PerCP-Cy5.5 (sc-6260 PCPC5) conjugate for intracellular FCM, 100 tests
  • agarose conjugate for IP studies, sc-6260 AC, 500 µg/0.25 ml agarose
  • Alexa Fluor® 405 (sc-6260 AF405), Alexa Fluor® 488 (sc-6260 AF488) and Alexa Fluor® 647 (sc-6260 AF647) conjugates for FCM or immunofluorescence; 100 µg/2 ml
  • See 80 citations for Vimentin Antibody (V9)
 

See additional Vimentin Antibodies.

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 Ordering Information
Product NameCatalog #UnitPriceQtyAdd 
Vimentin Antibody (V9) sc-6260 200 µg/ml $279
Vimentin Antibody (V9) AC sc-6260 AC 500 µg/ml, 25% ag $366
Vimentin Antibody (V9) Alexa Fluor® 405 sc-6260 AF405 100 µg/2 ml $314
Vimentin Antibody (V9) Alexa Fluor® 488 sc-6260 AF488 100 µg/2 ml $314
Vimentin Antibody (V9) Alexa Fluor® 647 sc-6260 AF647 100 µg/2 ml $314
Vimentin Antibody (V9) FITC sc-6260 FITC 200 µg/ml $282
Vimentin Antibody (V9) PCPC5 sc-6260 PCPC5 100 tests in 2 ml $310
Vimentin Antibody (V9) PE sc-6260 PE 100 tests in 2 ml $303
Vimentin Antibody (V9) PerCP sc-6260 PerCP 100 tests in 2 ml $310
Vimentin Antibody (V9) TRITC sc-6260 TRITC 200 µg/ml $282
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
Vimentin siRNA (h) sc-29522 10 µM $258
Vimentin siRNA (r) sc-156015 10 µM $258
Vimentin (h)-PR sc-29522-PR 10 µM, 20 µl $23
Vimentin (r)-PR sc-156015-PR 10 µM, 20 µl $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
Vimentin shRNA Plasmid (h) sc-29522-SH 20 µg $520
Vimentin shRNA Plasmid (r) sc-156015-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
Vimentin shRNA (h) Lentiviral Particles sc-29522-V 200 µl $625
Vimentin shRNA (r) Lentiviral Particles sc-156015-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
A-10 Cell Lysate sc-3806 500 µg/200 µl $104
KNRK Whole Cell Lysate sc-2214 500 µg/200 µl $104
HISM Cell Lysate sc-2229 500 µg/200 µl $104
HeLa Whole Cell Lysate sc-2200 500 µg/200 µl $104
PC-12 Cell Lysate sc-2250 500 µg/200 µl $104
SJRH30 Cell Lysate sc-2287 500 µg/200 µl $104
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human VIM 7431 10p12.33 NM_003380 P08670
 193060
 

Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. Intermediate filaments are constructed from two-chain, a-helical, coiled-coil molecules arranged on an imperfect helical lattice and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. One such intermediate filament protein, Vimentin, is a general marker of cells originating in the mesenchyme. Vimentin is frequently coexpressed with other members of the intermediate filament family, such as the cytokeratins, in neoplasms including melanoma and breast carcinoma.

Vimentin Antibody (V9) Product Citations
See how others have used Vimentin Antibody (V9) and or Vimentin Antibody (V9) conjugates.


80 total citations
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Vimentin Antibody (V9) Data
Click on image to enlarge
sc-6260
Vimentin (V9): sc-6260. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining.
sc-6260
Vimentin (V9): sc-6260. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoskeletal localization.
sc-6260
Vimentin (V9) PE: sc-6260 PE. Intracellular FCM analysis of fixed and permeabilized PC-12 cells. Black line histogram represents the isotype control, normal mouse IgG1: sc-2866.
sc-6260
Western blot analysis of Vimentin expression in A-10 (A,C) and KNRK (B) whole cell lysates. Antibodies tested include Vimentin (V9): sc-6260 (A,B) and Vimentin (C-20): sc-7557 (C).
sc-6260
Vimentin (V9) Alexa Fluor 488: sc-6260 AF488. Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal mouse IgG1: sc-3890.
sc-6260
Vimentin (V9) Alexa Fluor 647: sc-6260 AF647. Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal mouse IgG1: sc-24636.
sc-6260
Vimentin (V9): sc-6260.Immunofluorescence staining of methanol-fixed HeLa cells showing cytoskeletal localization using indirect FITC (A) staining and direct Alexa Fluor 488 (B) staining.
sc-6260
Vimentin (V9) FIT: sc-6260 FITC. Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal mouse IgG1: sc-2855.
sc-6260
Western blot analysis of Vimentin phosphorylation in untreated (A,D), paclitaxel treated (B,E) and paclitaxel and lambda protein phosphatase (sc-200312A) treated (C,F) Jurkat whole cell lysates. Antibodies tested include p-Vimentin (Ser 83): sc-130610 (A,B,C) and Vimentin (V9): sc-6260 (D,E,F).
sc-6260
Vimentin (V9): sc-6260. Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing cytoskeletal staining of cells in epididymal ducts.
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