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- mouse monoclonal IgG1; 200 µg/ml
- recommended for detection of JNK1, JNK2 and JNK3 of mouse, rat and human origin phosphorylated at Thr 183 and Tyr 185 by WB, IP, IF, IHC(P) and ELISA
- agarose conjugate for IP studies, sc-6254 AC, 500 µg/0.25 ml agarose
- fluorescein (sc-6254 FITC) and rhodamine (sc-6254 TRITC) conjugates for immunofluorescence, 200 µg/ml
- blocking peptide, sc-6254 P
- Alexa Fluor® 405 (sc-6254 AF405), Alexa Fluor® 488 (sc-6254 AF488) and Alexa Fluor® 647 (sc-6254 AF647) conjugates for immunofluorescence; 100 µg/2 ml
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JNK Background Information
The mitogen-activated protein (MAP) kinases ERK 1 and ERK 2 are proline-directed kinases that are activated through concomitant phosphorylation of tyrosine and threonine residues (1,2). The JNK family, which includes JNK1, JNK2, and JNK3, is distantly related to the MAP kinase family, members of which are activated by dual phosphorylation at a Thr-Pro-Tyr motif, specifically at Thr-183 and Tyr-185 residues, in response to ultraviolet (UV) light (3-6). This motif is divergent from the Thr-Glu-Tyr motif characteristic of the MAP kinase family (6). JNK is phosphorylated by JNK-activating kinase (JNKK1 and JNKK2), which are members of the MEK family (7). Activated JNK mediates the phosphorylation of c-Jun at the amino terminal serine regulatory sites, Ser-63 and Ser-73, which stimulates the transactivation function of c-Jun (5,6).
| p-JNK (G-7) Product Citations |
See how others have used p-JNK (G-7): sc-6254 antibody and or p-JNK (G-7) antibody conjugates.
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p-JNK (G-7)
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Western blot analysis of control (A,C,E,G) and UV-treated (B,D,F,H) JNK1 (A,B,E,F) and JNK2 (C,D,G,H) transfected COS cells. Antibodies tested include p-JNK (G-7): sc-6254 (A-D) and JNK1 (FL): sc-571 (E-H). p-JNK (G-7): sc-6254. Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human skin showing nuclear staining of epidermal cells.
Western blot analysis of inactive His-tagged human recombinant JNK p46 (A,C) and His-tagged human recombinant JNK p46 phosphorylated by human recombinant MKK4 (B,D). Antibodies tested include JNK1/3 (C-17): sc-474 (A,B) and p-JNK (G-7): sc-6254 (C,D). Western blot analysis of JNK phosphorylation in untreated (A,D), anisomycin treated (B,E) and anisomycin and lambda protein phosphatase ( sc-200312A) treated (C,F) NIH/3T3 whole cell lysates. Antibodies tested include p-JNK (G-7): sc-6254 (A,B,C) and JNK (D-2): sc-7345 (D,E,F). Western blot analysis of JNK phosphorylation in untreated (A,D), UV treated (B,E) and UV and lambda protein phosphatase ( sc-200312A) treated (C,F) HeLa whole cell lysates. Antibodies tested include p-JNK (G-7): sc-6254 (A,B,C) and JNK (D-2): sc-7345 (D,E,F). |
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