Cdk5 (J-3) Antibody sc-6247

santa cruz biotechnology, inc.

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Cdk5 (J-3) Antibody: sc-6247	\| Datasheet

mouse monoclonal IgG₁; 200 µg/ml
raised against amino acids 1-291 representing full length Cdk5 of human origin
recommended for detection of Cdk5 of mouse, rat and human origin by WB, IP, IF and ELISA
agarose conjugate for IP studies, sc-6247 AC, 500 µg/0.25 ml agarose

Additional Cdk Antibodies ...

Ordering InformationProduct Citations

Recommended Support Products:

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WB IP IF siRNA

Species	Gene Name	Gene ID	Chromosome Location	Isoform (mRNA) Accession #	Protein Accession #	OMIM™ Number

Human	CDK5	1020	7q36.1	NM_004935	Q00535	123831
Mouse	Cdk5	12568	5 A3		P49615	N/A

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Ordering Information

Product Name	Catalog #	Unit	Price	Qty	Add	Favorites
Cdk5 (J-3)	sc-6247	200 µg/ml	$279
Cdk5 (J-3) AC	sc-6247 AC	500µg/ml, 25%ag	$366

siRNA Gene Silencers (click product name for more information)

Product Name	Catalog #	Unit	Price	Qty	Add	Favorites
Cdk5 siRNA (m)	sc-35047	10 µM	$258
Cdk5 (m)-PR	sc-35047-PR	10 µM	$23

shRNA Plasmids (click product name for more information)

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shRNA Lentiviral Particles (click product name for more information)

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WB Positive Control Cell Lysates (click product name for more information)

Product Name	Catalog #	Unit	Price
HeLa + PMA nuclear extract	sc-2121	1000 µg	$143
K-562 + PMA nuclear extract	sc-2131	1000 µg	$143
Jurkat + PMA nuclear extract	sc-2133	1000 µg	$143
SK-BR-3 + PMA nuclear extract	sc-2135	1000 µg	$143
HeLa nuclear extract	sc-2120	1000 µg	$143
K-562 nuclear extract	sc-2130	1000 µg	$143
Jurkat nuclear extract	sc-2132	1000 µg	$143
SK-BR-3 nuclear extract	sc-2134	1000 µg	$143

Cdk5 Background Information
Cell cycle progression is controlled in part by a family of cyclin proteins and cyclin dependent kinases (Cdks). Cdk proteins work in concert with the cyclins to phosphorylate key substrates involved in each phase of cell cycle progression. Another family of proteins, Cdk inhibitors, also plays a role in regulating cell cycle by binding to cyclin-Cdk complexes and modulating their activity. Several Cdk proteins have been identified, including Cdk2-Cdk8, PCTAIRE-1–3, PITALRE and PITSLRE. Cdk5 is thought to be involved in the G1-S transition of the cell cycle and is highly expressed in mature neurons. Activity of Cdk5 increases significantly during neuronal differentiation. Cdk5 has been postulated to be a neurofilament or tau protein kinase, based on its ability to phosphorylate these proteins in vitro.

Cdk5 (J-3) Product Citations

See how others have used Cdk5 (J-3): sc-6247 antibody and or Cdk5 (J-3) antibody conjugates.

19 total citations

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Cdk5 (J-3)

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Cdk5 (J-3): sc-6247. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.

Cdk5 (J-3): sc-6247. Western blot analysis of Cdk5 expression in HeLa (A), K-562 (B), Jurkat (C) and SK-BR-3 (D) whole cell lysates.

Western blot detection of Cdk5 phosphorylation in untreated (A,C) and EGF-treated A-431 cultures (B,D). Blots were probed with Cdk5 (J-3): sc-6247 (A,B) and p-Cdk5 (Tyr 15): sc-12918 (C,D).

Cdk5 (J-3): sc-6247. Western blot analysis of Cdk5 expression in NIH/3T3 (A), RAW 264.7 (B), MM-142 (C) and WEHI-231 (D) nuclear extracts.

Western blot analysis of Cdk5 phosphorylation in untreated (A,D), nocodazole treated (B,E) and lambda protein phosphatase and nocodazole treated (C,F) SK-N-SH whole cell lysate. Antibodies tested include p-Cdk5 (Ser 159)-R: sc-12919-R (A,B,C) and Cdk5 (J-3): sc-6247 (D,E,F).

Western blot analysis of Cdk5 phosphorylation in untreated (A,D), nocodazole treated (B,E) and nocodazole and lambda protein phosphatase (sc-200312A) treated (C,F) SK-N-SH whole cell lysates. Antibodies tested include p-Cdk5 (B-4): sc-271981 (A,B,C) and Cdk5 (J-3): sc-6247 (D,E,F).

bovine anti-mouse IgG-HRP: sc-2375. Western blot analysis of Cdk5 expression in HeLa whole cell lysate. Antibody tested: Cdk5 (J-3): sc-6247

chicken anti-mouse IgG-HRP: sc-2954. Western blot analysis of Cdk5 expression in HeLa whole cell lysate. Antibody tested: Cdk5 (J-3): sc-6247.

goat anti-mouse IgG, F(ab')₂-HRP: sc-3697. Western blot analysis of Cdk5 expression in HeLa whole cell lysate. Antibody tested: Cdk5 (J-3): sc-6247.

bovine anti-mouse IgG-HRP: sc-2371. Western blot analysis of Cdk5 expression in HeLa whole cell lysate. Antibody tested: Cdk5 (J-3): sc-6247.

rabbit anti-mouse IgG-HRP: sc-358914. Western blot analysis of Cdk5 expression in HeLa whole cell lysate. Antibody tested: Cdk5 (J-3): sc-6247.

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Cdk5 (J-3) Antibody: sc-6247