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- rabbit polyclonal IgG, 200 µg/ml
- epitope corresponding to amino acids 1-96 mapping at the N-terminus of ATF-2 of human origin
- recommended for detection of ATF-2 of mouse, rat, human and Xenopus origin by WP, IP, IF, IHC(P) and ELISA; also reactive with additional species, including equine, canine, bovine, porcine and avian
- TransCruz reagent for Gel Supershift and ChIP applications, sc-6233 X, 200 µg/0.1 ml
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Ordering InformationProduct Citations
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| Species |
Gene Name |
Gene ID |
Chromosome Location |
Isoform (mRNA) Accession # |
Protein Accession # |
OMIM™ Number |
| Human |
ATF2 |
1386 |
2q31.1 |
NM_001880 |
P15336
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123811 |
| Mouse |
Atf2 |
11909 |
2 C3 |
|
P16951
|
N/A |
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ATF-2 Background Information
Eukaryotic gene transcription is regulated by sequence-specific transcription factors which bind modular cis-acting promotor and enhancer elements. The ATF/CREB transcription factor family binds the palindromic cAMP response element (CRE) octanucleotide TGACGTCA. The ATF/CREB family includes CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. This family of proteins contain highly divergent N-terminal domains, but share a C-terminal leucine zipper for dimerization and DNA binding. ATF-2 forms homodimers and heterodimers with c-Jun to initiate CRE-dependent transcription. Phosphorylation of ATF-2 at Thr 69 and Thr 71 by stress-activated kinases is necessary for transcriptional activation. Myc also induces phosphorylation of ATF-2 at Thr 69 and Thr 71 to prolong the half-life of ATF-2. ATF-2 also functions as a histone acetyltransferase (HAT) by specifically acetylating histones H2B and H4 in vitro. The gene encoding human ATF-2 maps to chromosome 2q31.1.
| ATF-2 (N-96) Product Citations |
See how others have used ATF-2 (N-96): sc-6233 antibody and or ATF-2 (N-96) antibody conjugates.
30 total citations Loading citations.
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ATF-2 (N-96)
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ATF-2 (N-96): sc-6233. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human tonsil showing nuclear localization.
Western blot analysis of human recombinant ATF-2 untreated fusion protein (A,C) or phosphorylated by human recombinant JNK1 (B,D). Antibodies tested include ATF-2 (N-96): sc-6233 (A,B) and p-ATF-2 (Thr 71)-R: sc-7982-R (C,D). ATF-2 (N-96): sc-6233. Western blot analysis of ATF-2 expression in K-562 (A) and A-673 (B) nuclear extracts.
ATF-2 siRNA (h): sc-29205. Western blot analysis of ATF-2 expression in non-transfected control (A) and ATF-2 siRNA transfected (B) HeLa cells. Blot probed with ATF-2 (N-96): sc-6233. Lamin A/C (H-110): sc-20681 used as specificity and loading control. ChIP analysis of transcription factor binding to the Interferon β promoter before (A) and six hours after (B) Sendai virus infection of HeLa cells. Antibodies tested included NFκB p65 (A): sc-109, ATF-2 (N-96): sc-6233, GCN5 (N-18): sc-6303, Pol II (H-224): sc-9001, TFIID (TBP)(SI-1): sc-273 and CBP (A-22): sc-369. Data kindly provided by G. Mosialos. ATF-2 (N-96): sc-6233. Western blot analysis of ATF-2 expression in HeLa whole cell lysate (A) and A-673 (B) nuclear extract.
ATF-2 (N-96): sc-6233. Western blot analysis of ATF-2 expression in non-transfected 293T: sc-117752 (A), mouse ATF-2 transfected 293T: sc-126459 (B) and HeLa (C) whole cell lysates. |
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