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Lamin B Antibody (M-20): sc-6217

 |  Datasheet

(Based on data analysis)

  • Lamin B Antibody (M-20) is a goat polyclonal IgG provided at 200 µg/ml
  • epitope mapping at the C-terminus of Lamin B1 of mouse origin
  • recommended for detection of Lamin B1 and, to a lesser extent, Lamin B2 of mouse, rat and human origin by WB, IP, IF, IHC(P), ELISA and indirect FCM using PE (sc-3743)- or FITC (sc-2024)-conjugated donkey anti-goat IgG
  • blocking peptide, sc-6217 P
  • See 133 citations for Lamin B Antibody (M-20)
 

See additional Lamin Antibodies including Lamin, Lamin A, Lamin B, Lamin B1, Lamin C and Lamin B2.

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 Ordering Information
Product NameCatalog #UnitPriceQtyAdd 
Lamin B Antibody (M-20) sc-6217 200 µg/ml $279
Lamin B (M-20) P sc-6217 P
(peptide)
 100 µg/0.5 ml $61
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
Lamin B1 siRNA (h) sc-29386 10 µM $258
Lamin B1 siRNA (m) sc-35779 10 µM $258
Lamin B (h)-PR sc-29386-PR 10 µM, 20 µl $23
Lamin B1 (m)-PR sc-35779-PR 10 µM, 20 µl $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
Lamin B shRNA Plasmid (h) sc-29386-SH 20 µg $520
Lamin B1 shRNA Plasmid (m) sc-35779-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
Lamin B shRNA (h) Lentiviral Particles sc-29386-V 200 µl $625
Lamin B1 shRNA (m) Lentiviral Particles sc-35779-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
Jurkat Whole Cell Lysate sc-2204 500 µg/200 µl $104
Y79 Cell Lysate sc-2240 500 µg/200 µl $104
HeLa Whole Cell Lysate sc-2200 500 µg/200 µl $104
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human LMNB1 4001 5q23.2 NM_005573 P20700
 169500
Mouse Lmnb1 16906 18 D3 P14733
 N/A
 

A unique family of cysteine proteases has been described that differs in sequence, structure and substrate specificity from any previously described protease family. This family, termed Ced-3/ICE, functions as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity. Nuclear lamins are critical to maintaining the integrity of the nuclear envelope and cellular morphology as components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations. Nuclear Lamin B is fragmented as a consequence of apoptosis by an unidentified member of the ICE family.

Lamin B Antibody (M-20) Product Citations
See how others have used Lamin B Antibody (M-20) and or Lamin B Antibody (M-20) conjugates.


133 total citations
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Lamin B Antibody (M-20) Data
Click on image to enlarge
sc-6217
Western blot analysis of Lamin B expression in F9 (A), Jurkat (B,D) and Y79 (C) whole cell lysates. Antibodies tested include Lamin B (C-20): sc-6216 (A,B) and Lamin B (M-20): sc-6217 (C,D).
sc-6217
Lamin B (M-20): sc-6217. Immunofluorescence staining of methanol-fixed Jurkat cells showing staining of nuclear lamina.
sc-6217
Lamin B (M-20): sc-6217. Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human tonsil showing localization to the nuclear lamina.
sc-6217
Lamin B (M-20): sc-6217. Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with Lamin B (M-20), followed by FITC-conjugated donkey anti-goat IgG: sc-2024. Black line histogram represents the isotype control, normal goat IgG: sc-3887.
sc-6217
Lamin B (M-20): sc-6217. Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with Lamin B (M-20), followed by PE-conjugated donkey anti-goat IgG: sc-3743. Black line histogram represents the isotype control, normal goat IgG: sc-3887.
sc-6217
Lamin B (M-20): sc-6217. Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with Lamin B (M-20), followed by PE-Cy5-conjugated donkey anti-goat IgG F(ab')2: sc-3858. Black line histo-gram represents the isotype control, normal goat IgG: sc-3887.
sc-6217
Lamin B (M-20): sc-6217. Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with Lamin B (M-20), followed by PE-conjugated rabbit anti-goat IgG: sc-3755. Black line histogram represents the isotype control, normal goat IgG: sc-3887.
sc-6217
Lamin B (M-20): sc-6217. Western blot analysis of Lamin B1 expression in non-transfected 293T: sc-117752 (A), human Lamin B1 transfected 293T: sc-111764 (B) and Y79 (C) whole cell lysates.
sc-6217
Lamin B (M-20): sc-6217. Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with Lamin B (M-20), followed by PE-conjugated donkey anti-goat IgG F(ab')2: sc-3857. Black line histogram represents the isotype control, normal goat IgG: sc-3887.
sc-6217
Lamin B (M-20): sc-6217. Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with Lamin B (M-20), followed by FITC-conjugated chicken anti-goat IgG: sc-2988. Black line histogram represents the isotype control, normal goat IgG: sc-3887.
sc-6217
Lamin B1 siRNA (h): sc-29386. Western blot analysis of Lamin B1 expression in non-transfected control (A) and Lamin B1 siRNA transfected (B) HeLa cells. Blot probed with Lamin B (M-20): sc-6217.
sc-6217
Lamin B (M-20): sc-6217. Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with Lamin B (M-20), followed by FITC-conjugated mouse anti-goat IgG: sc-2356. Black line histogram represents the isotype control, normal goat IgG: sc-3887.
sc-6217
Lamin B (M-20): sc-6217. Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with Lamin B (M-20), followed by FITC-conjugated rabbit anti-goat IgG: sc-2777. Black line histogram represents the isotype control, normal goat IgG: sc-3887.
sc-6217
Lamin B (M-20): sc-6217. Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with Lamin B (M-20), followed by PE-conjugated mouse anti-goat IgG: sc-3752. Black line histogram represents the isotype control, normal goat IgG: sc-3887.
sc-6217
Lamin B (M-20): sc-6217. Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with Lamin B (M-20), followed by PE-conjugated chicken anti-goat IgG: sc-3760. Black line histogram represents the isotype control, normal goat IgG: sc-3887.
sc-6217
Lamin B (M-20): sc-6217. Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with Lamin B (M-20), followed by FITC-conjugated bovine anti-goat IgG: sc-2348. Black line histogram represents the isotype control, normal goat IgG: sc-3887.
sc-6217
Lamin B (M-20): sc-6217. Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with Lamin B (M-20), followed by PE-conjugated bovine anti-goat IgG: sc-3747. Black line histogram represents the isotype control, normal goat IgG: sc-3887.
sc-6217
Lamin B (M-20): sc-6217. Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with Lamin B (M-20), followed by PerCP-conjugated donkey anti-goat IgG: sc-45091. Black line histogram represents the isotype control, normal goat IgG: sc-3887.
sc-6217
Lamin B (M-20): sc-6217. Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with Lamin B (M-20), followed by PerCP-conjugated bovine anti-goat IgG: sc-45096. Black line histogram represents the isotype control, normal goat IgG: sc-3887.
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