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western blotting image from recommended IRF-4 (F-4) monoclonal antibody replacement. Image 398865
This polyclonal antibody has been discontinued. See our recommended IRF-4 (F-4) monoclonal antibody (sample data shown).
western blotting image from recommended IRF-4 (F-4) monoclonal antibody replacement. Image 398865
This polyclonal antibody has been discontinued. See our recommended IRF-4 (F-4) monoclonal antibody (sample data shown).
This polyclonal antibody has been discontinued. See our recommended IRF-4 (F-4) monoclonal antibody (sample data shown).

IRF-4 Antibody (M-17): sc-6059

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  • IRF-4 Antibody (M-17) is a goat polyclonal IgG; 100 µg/ml
  • epitope mapping at the C-terminus of IRF-4 of mouse origin
  • Discontinued polyclonal antibody

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Description

SEE ALSO...

Additional IRF Antibodies including IRF-1, IRF-2, IRF-3, IRF-4, IRF-5, IRF-6, IRF-7, IRF-2BP1 and IRF-2BP

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

Ordering Information

IRF-4 (F-4): sc-48338 [ Recommended monoclonal replacement for IRF-4 (M-17) ]

Product NameCatalog #UNITPriceQtyFAVORITES

IRF-4 Antibody (F-4)

sc-48338
200 µg/ml
$316.00

IRF-4 Antibody (F-4): m-IgG Fc BP-HRP Bundle

sc-525558
200 µg Ab; 10 µg BP
$354.00

IRF-4 Antibody (F-4): m-IgG2a BP-HRP Bundle

sc-546314
200 µg Ab; 10 µg BP
$354.00

IRF-4 Antibody (F-4) X

sc-48338 X
200 µg/0.1 ml
$316.00

IRF-4 Antibody (F-4) AC

sc-48338 AC
500 µg/ml, 25% agarose
$416.00

IRF-4 Antibody (F-4) HRP

sc-48338 HRP
200 µg/ml
$316.00

IRF-4 Antibody (F-4) FITC

sc-48338 FITC
200 µg/ml
$330.00

IRF-4 Antibody (F-4) PE

sc-48338 PE
200 µg/ml
$343.00

IRF-4 Antibody (F-4) Alexa Fluor® 488

sc-48338 AF488
200 µg/ml
$357.00

IRF-4 Antibody (F-4) Alexa Fluor® 546

sc-48338 AF546
200 µg/ml
$357.00

IRF-4 Antibody (F-4) Alexa Fluor® 594

sc-48338 AF594
200 µg/ml
$357.00

IRF-4 Antibody (F-4) Alexa Fluor® 647

sc-48338 AF647
200 µg/ml
$357.00

IRF-4 Antibody (F-4) Alexa Fluor® 680

sc-48338 AF680
200 µg/ml
$357.00

IRF-4 Antibody (F-4) Alexa Fluor® 790

sc-48338 AF790
200 µg/ml
$357.00

I was planning to purchase the "IRF-4 Antibody (M-17): sc-6059" for a CUT&RUN experiment, but I’ve discovered that it has been discontinued. Could you recommend any alternative antibodies that would be suitable for this application?

Asked by: Ya J
Thank you for your question.We have not validated our antibodies for CUT&RUN experiments. Here is the alternative replacement : https://www.scbt.com/p/irf-4-antibody-f-4
Answered by: BlakeJ
Date published: 2024-08-12

Dear representative, is it possible to receive the datasheet of this product since we have it still in our lab. Additionally I would like to ask, which specific IgG subtype the antibody was (like IgG1, IgG2) Thank you

Asked by: Naal
Thank you for your question. You can still download a PDF of the product data sheet on our website using the Datasheet Search at the bottom of our website under Resources (see link provided below). This is a goat polyclonal antibody and contains a mixture of IgG subtypes. https://www.scbt.com/scbt/resources/datasheet-search
Answered by: Technical Support
Date published: 2019-02-27

What is a starting dilution for IRF-4 to stain mouse tissues by IHC (paraffin embedded). Thanks.

Asked by: Penny
Thank you for your question. We recommend a starting dilution of 1:50 (range of 1:50-1:500) for IHC(P). A link to our recommended protocol is below. https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining Please note that this goat polyclonal antibody (sc-6059) is now discontinued. For a limited time, we are offering free 10µg sample sizes of our highly recommended mouse monoclonal replacement, IRF-4 Antibody (F-4): sc-48338. (https://www.scbt.com/scbt/product/irf-4-antibody-f-4). Please contact our Technical Service Department if you would like to test a sample.
Answered by: Technical Support
Date published: 2017-03-02
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Rated 5 out of 5 by from Used in WB and correct MW band at 1:200 dilutionUsed in WB and correct MW band at 1:200 dilution.
Date published: 2015-07-01
Rated 5 out of 5 by from Several publications describe success inSeveral publications describe success in Western Blot with this antibody using human transfected Jurkat cells, T-lymphocytes, KARPAS 299, K-562, U-937, 293T and B cells. -SCBT Publication Review
Date published: 2015-04-22
Rated 4 out of 5 by from A definite increase in signal can be observedA definite increase in signal can be observed in 293T cells that have been transfected to over-express the human IRF-4 protein when compared to non-transfected 293T cells by western blot. -SCBT QC
Date published: 2015-04-21
Rated 5 out of 5 by from Works well for ChIP application using humanWorks well for ChIP application using human and mouse T-cells, 3T3-L1 cells, RAW cells. -SCBT Publication Review
Date published: 2015-04-11
Rated 5 out of 5 by from Antibody scAntibody sc-6059 successfully used to show an increase in expression of IRF-4 when activated using the IRF-4 CRISPR Activation Plasmid by Western Blot. -SCBT QC
Date published: 2015-04-08
Rated 5 out of 5 by from Published data for coPublished data for co-immunoprecipitation using S194,RAMOS, RAJI, and BJAB,293,BOSC cells,NIH 3T3 cells. -SCBT Publication Review
Date published: 2015-04-03
Rated 5 out of 5 by from This antibody was reported to be successfulThis antibody was reported to be successful in EMSA application using human DLD-1, HT29-18N2, U937, Jurkat, Raji, HeLa, SB cells and mouse NIH/3T3, RAW 264.7 cells. -SCBT Publication Review
Date published: 2015-03-25
Rated 5 out of 5 by from Researchers have reported success in flowResearchers have reported success in flow cytometry using PT and BR cell lines, JEKO, HBL2, MINO, and REC1 PT cell lines, H929, OCI-LY19 cell lines. -SCBT Publication Review
Date published: 2015-03-06
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IRF-4 Antibody (M-17) is rated 4.9 out of 5 by 11.
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