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- rabbit polyclonal IgG, 200 µg/ml; also available as goat IgG, 200 µg/ml, sc-601-G
- epitope mapping at the C-terminus of Cdk4 of human origin
- recommended for detection of Cdk4 of mouse, rat and human origin by WB, IP, KA, IF and ELISA; also reactive with additional species, including equine, canine, bovine and porcine
- blocking peptide, sc-601 P
- agarose conjugate for IP studies, sc-601 AC, 500 µg/0.25 ml agarose
- HRP conjugate, sc-601 HRP, 200 µg/ml
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Ordering InformationProduct Citations
Recommended Support Products:
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| Species |
Gene Name |
Gene ID |
Chromosome Location |
Isoform (mRNA) Accession # |
Protein Accession # |
OMIM™ Number |
| Human |
CDK4 |
1019 |
12q14.1 |
NM_000075 |
P11802
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609048 |
| Mouse |
Cdk4 |
12567 |
10 D3 |
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P30285
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N/A |
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Cdk4 Background Information
Cell cycle progression is controlled in part by a family of cyclin proteins and cyclin dependent kinases (Cdks). Cdk proteins work in concert with the cyclins to phosphorylate key substrates involved in each phase of cell cycle progression. Another family of proteins, Cdk inhibitors, also plays a role in regulating the cell cycle by binding to cyclin-Cdk complexes and modulating their activity. Several Cdk proteins have been identified, including Cdk2-Cdk8, PCTAIRE-1-PCTAIRE-3, PITALRE and PITSLRE. Cdk4, in complex with D-type cyclins, is thought to regulate cell growth during the G1 phase of the cell cycle. This association with a D-type cyclin upregulates Cdk4 activity, whereas binding to the Cdk inhibitor p16 downregulates Cdk4 activity. Activation of the Cdk4-cyclin complexes requires phosphorylation on a single threonyl residue of Cdk4, catalyzed by a Cdk-activating protein (CAK).
| Cdk4 (H-22) Product Citations |
See how others have used Cdk4 (H-22): sc-601 antibody and or Cdk4 (H-22) antibody conjugates.
125 total citations Loading citations.
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Cdk4 (H-22)
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Cdk4 (H-22)-G: sc-601-G. Western blot analysis of Cdk4 expression in HeLa (A), Ramos (B) and K-562 (C) whole cell lysates. Cdk4 (H-22)-G: sc-601-G. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear staining. Cdk4 (H-22): sc-601. Western blot analysis of Cdk4 expression in non-transfected 293T: sc-117752 (A), mouse Cdk4 transfected 293T: sc-119150 (B) and HeLa (C) whole cell lysates. Cdk4 (H-22)-G: sc-601-G. Western blot analysis of Cdk4 expression in non-transfected 293T: sc-117752 (A), mouse Cdk4 transfected 293T: sc-119150 (B) and HeLa (C) whole cell lysates. Cdk4 (H-22): sc-601. Western blot analysis of Cdk4 expression in Ramos whole cell lysate.
Cdk4 (H-22)-G: sc-601-G. Western blot analysis of Cdk4 expression in Ramos whole cell lysate. donkey anti-goat IgG-HRP: sc-2033. Western blot analysis of Cdk4 expression in HeLa whole cell lysate. Antibody tested: Cdk4 (H-22)-G: sc-601-G. donkey anti-goat IgG-HRP: sc-2304. Western blot analysis of Cdk4 expression in Hela whole cell lysate. Antibody tested: Cdk4 (H-22)-G: sc-601-G. bovine anti-goat IgG-HRP: sc-2378. Western blot analysis of Cdk4 expression in HeLa whole cell lysate. Antibody tested: Cdk4 (H-22)-G: sc-601-G. bovine anti-goat IgG-HRP: sc-2384. Western blot analysis of Cdk4 expression in HeLa whole cell lysate. Antibody tested: Cdk4 (H-22)-G: sc-601-G. |
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