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- rabbit polyclonal IgG, 200 µg/ml; also available as goat IgG, 200 µg/ml, sc-59-G
- epitope corresponding to amino acids 528-546 mapping within an internal region of Sp1 of rat origin
- recommended for detection of both p95 and p106 Sp1 proteins of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine, bovine, porcine and avian
- blocking peptide, sc-59 P
- TransCruz reagent for Gel Supershift and ChIP applications, sc-59 X, 200 µg/0.1 ml
- agarose conjugate for IP studies, sc-59 AC, 500 µg/0.25 ml agarose
- fluorescein (sc-59 FITC) and rhodamine (sc-59 TRITC) conjugates for immunofluorescence, 200 µg/ml
- Alexa Fluor® 405 (sc-59 AF405), Alexa Fluor® 488 (sc-59 AF488) and Alexa Fluor® 647 (sc-59 AF647) conjugates for immunofluorescence; 100 µg/2 ml
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Ordering InformationProduct Citations
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| Species |
Gene Name |
Gene ID |
Chromosome Location |
Isoform (mRNA) Accession # |
Protein Accession # |
OMIM™ Number |
| Human |
SP1 |
6667 |
12q13.13 |
NM_138473 |
P08047
|
189906 |
| Mouse |
Sp1 |
20683 |
15 F3 |
|
O89090
|
N/A |
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Sp1 Background Information
Sp1 is a sequence-specific transcription factor that recognizes GGGGCGGGGC and closely related sequences, which are often referred to as GC boxes. Sp1 was initially identified as a HeLa cell-derived factor that selectively activates in vitro transcription from the SV40 promoter and binds to the multiple GC boxes in the 21-bp repeated elements in SV40. The sequence specificity of DNA binding is conferred by Zn (II) fingers, whereas a different region of Sp1 appears to regulate the affinity of DNA binding. Sp1 belongs to a subgroup of transcription factors that are phosphorylated upon binding to promoter sequences. Evidence suggests that the early growth response gene, Erg-1 (also known as Zif268 or NGF1-A) (7), may downregulate certain mammalian gene promoters by competing with Sp1 for binding to an overlapping binding motif. The gene encoding human Sp1 maps to chromosome 12q13.1.
| Sp1 (PEP 2) Product Citations |
See how others have used Sp1 (PEP 2): sc-59 antibody and or Sp1 (PEP 2) antibody conjugates.
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Sp1 (PEP 2)
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Sp1 (PEP 2)-G: sc-59-G. Western blot analysis of Sp1 expression in K-562 (A,B) and Jurkat (C,D) nuclear extracts (A,C) and whole cell lysates (B,D). Sp1 (PEP 2)-G: sc-59-G. Immunofluorescence staining of methanol-fixed NIH/3T3 cells (A) and immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon carcinoma tissue (B) showing nuclear localization. Sp1 (PEP 2): sc-59. Western blot analysis of Sp1 expression in HeLa (A), K-562 (B) and A-431 (C) whole cell lysates.
Sp1 (PEP 2): sc-59. Immunofluorescence staining of methanol-fixed KNRK cells showing nuclear localization.
Sp1 siRNA (h): sc-29487. Western blot analysis of Sp1 expression in non-transfected control (A) and Sp1 siRNA transfected (B) A-431 cells. Blot probed with Sp1 (PEP 2): sc-59. α-actinin (H-2): sc-17829 used as specificity and loading control. Sp1 siRNA (h): sc-29487. Immunofluorescence staining of methanol-fixed, control HeLa (A) and Sp1 siRNA silenced HeLa (B) cells showing diminished nuclear staining in the siRNA silenced cells. Cells probed with Sp1 (PEP 2): sc-59. Sp1 (PEP-2): sc-59. ChIP analysis of Sp1 recruitment to genomic amplicons. Four (1-4) different human genomic Sp-1and two control amplicons (5,6) were analyzed by quantitative PCR (primer sequences available in on-line supplemental data). Data generated in collaboration with Drs. N. Trinklein and R. Myers, Stanford University (ENCODE Project).
Sp1 (PEP 2): sc-59. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing nuclear localization using indirect FITC (A) staining and HeLa cells using direct Alexa Fluor 488 (B) staining.
Sp1 (PEP 2): sc-59. Immunofluorescence staining of normal mouse intestine frozen section showing nuclear staining.
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