CD163 Antibody (ED2): sc-58965

CD163 Antibody (ED2) is a mouse monoclonal IgG1 kappa light chain antibody developed to target CD163 with exceptional accuracy in mouse and rat species, as demonstrated by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). CD163, also known as M130 or SCARI1, is a crucial macrophage-associated antigen belonging to the scavenger receptor cysteine-rich (SRCR) superfamily, characterized by multiple SRCR domains that facilitate binding to haptoglobin-hemoglobin complexes. The structural configuration of CD163, featuring a series of extracellular SRCR domains, a transmembrane region, and a cytoplasmic tail, is vital for mediating the endocytosis and clearance of hemoglobin-haptoglobin complexes, playing a significant role in anti-inflammatory response and iron metabolism. This precise structural arrangement allows CD163 to efficiently recognize and bind ligands in a calcium-dependent manner with high affinity, ensuring effective scavenging of free hemoglobin and preventing oxidative stress during hemolytic events. Additionally, various isoforms of CD163, which differ in cytoplasmic domains and potential phosphorylation sites, suggest diverse signaling mechanisms that regulate macrophage function and response to inflammatory stimuli. The membrane-bound localization of CD163 on macrophages and, to a lesser extent, on monocytes, is essential for interaction with extracellular ligands, facilitating cellular homeostasis and immune regulation. Anti-CD163 antibody (ED2) is available in both non-conjugated and multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, providing versatile options for diverse research applications.

The preferred CD163 antibody is CD163 Antibody (GHI/61): sc-20066.