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- rabbit polyclonal IgG, 200 µg/ml
- epitope mapping at the N-terminus of p300 of human origin
- recommended for detection of p300 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including equine, canine, porcine and avian
- blocking peptide, sc-584 P
- TransCruz reagent for Gel Supershift and ChIP applications, sc-584 X, 200 µg/0.1 ml
- agarose conjugate for IP studies, sc-584 AC, 500 µg/0.25 ml agarose
- fluorescein (sc-584 FITC) and rhodamine (sc-584 TRITC) conjugates for immunofluorescence, 200 µg/ml
- Alexa Fluor® 405 (sc-584 AF405), Alexa Fluor® 488 (sc-584 AF488) and Alexa Fluor® 647 (sc-584 AF647) conjugates for immunofluorescence; 100 µg/2 ml
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p300 Background Information
Cyclic AMP-regulated gene expression frequently involves a DNA element designated the cAMP-regulated enhancer (CRE). Many transcription factors bind to this element, including the protein CREB which is activated as a result of phosphorylation by protein kinase A. It has been shown that protein kinase A-mediated CREB phosphorylation results in its binding to a nuclear protein designated CBP (for CREB-binding protein). These findings suggest that CBP has many of the properties expected of a CREB co-activator. Another high molecular weight transcriptional adapter protein, designated p300, is characterized by three cysteine- and histidine-rich regions, of which the most carboxy terminal region specifically binds the adenovirus E1A protein. p300 molecules lacking an intact E1A binding site bypass E1A repression even in the presence of high concentrations of E1A. Sequence analysis of CBP and p300 has revealed substantial homology, arguing that these proteins are members of a conserved family of co-activators.
| p300 (N-15) Product Citations |
See how others have used p300 (N-15): sc-584 antibody and or p300 (N-15) antibody conjugates.
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p300 (N-15)
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p300 (N-15): sc-584. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear localization (A). Immunofluorescence staining of methanol-fixed SK-BR-3 cells showing nuclear localization (B).
p300 (N-15): sc-584. Western blot analysis of p300 expression in A-431 nuclear extract.
p300 siRNA (h): sc-29431. Western blot analysis of p300 expression in non-transfected control (A) and p300 siRNA transfected (B) A-431 cells. Blot probed with p300 (N-15): sc-584. Lamin A/C (636): sc-7292 used as specificity and loading control. ChIP analysis of coactivator recruitment on Cyclin D2 promoter in C2C12 cells treated with LiCl and serum. Antibodies tested include β-catenin (H-102): sc-7199, β-catenin (C-18): sc-1496, β-catenin (E-5): sc-7963, Tip60 (N-17): sc-5725, TRRAP (T-17): sc-5405, TRRAP (Y-18): sc-12375, TRRAP (F-20): sc-12376, TRRAP (H-300): sc-11411, CBP (A-22): sc-369, CBP (C-20): sc-583, CBP (451): sc-1211, CPB (C-1): sc-7300, p300 (H-272): sc-8981, p300 (N-15): sc-584 and p300 (C-20): sc-585. Data kindly provided by M.G. Rosenfeld and reproduced with permission from Kioussi et al., Cell 2002, 111: 673-685. ChIP analysis of cytokine gene promoter occupancy in TNFα-treated mouse embryonic fibroblasts. IP's permorned without (A) and with (B) primary antibody. Antibodies tested include NFκB p65 (A): sc-109, RelB (C-19): sc-226 and p300 (N-15): sc-584. Data kindly provided by A. Hoffmann. ChIP analysis of cofactor occupancy dynamics on the IL-8 promoter in 293 cells in response to IL-1β treatment. Antibodies tested include NFκB p50 (C-19): sc-1190, NFκB p50 (E-10): sc-8414, NFκB p50 (H-119): sc-7178, NFκB p65 (C-20): sc-372, NFκB p65 (A): sc-109, NFκB p65 (H-286): sc-7151, CBP (A-22): sc-369, CBP (C-1): sc-7300, CBP (C-20): sc-583, CBP (451): sc-1211, p300 (C-20): sc- sc-585, p300 (N-15): sc-584, p300 (H-272): sc-8981. Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67.
ChIP analysis of in vivo binding of RORα and its recruitment of coactivators to RORα-responsive promoters in freshly dissected cerebella derived from wild type (+/+) and staggerer (Sg) mice. Control Input (A). Antibodies used included TRβ1 (J51): sc-737 and TRβ1 (J52): sc-738 (B), p300 (C-20): sc-585, p300 (H-272): sc-8981 and p300 (N-15): sc-584 (C), β-catenin (H-102): sc-7199, β-catenin (C-18): sc-1496 and β-catenin (E-5): sc-7963 (D). Data kindly provided by M.G. Rosenfeld and reproduced wtih permission from Gold et al., Neuron 2003, 40: 1119-1131.
ChIP analysis of in vivo binding of RORα and its recruitment of coactivators to RORα-responsive promoters in freshly dissected cerebella derived from wild type (+/+) and staggerer (Sg) mice. Control Input (A). Antibodies used included TRβ1 (J51): sc-737 and TRβ1 (J52): sc-738 (B), p300 (C-20): sc-585, p300 (H-272): sc-8981 and p300 (N-15): sc-584 (C), β-catenin (H-102): sc-7199, β-catenin (C-18): sc-1496 and β-catenin (E-5): sc-7963 (D). Data kindly provided by M.G. Rosenfeld and reproduced wtih permission from Gold et al., Neuron 2003, 40: 1119-1131.
ChIP analysis of in vivo binding of RORα and its recruitment of coactivators to RORα-responsive promoters in freshly dissected cerebella derived from wild type (+/+) and staggerer (Sg) mice. Control Input (A). Antibodies used included TRβ1 (J51): sc-737 and TRβ1 (J52): sc-738 (B), p300 (C-20): sc-585, p300 (H-272): sc-8981 and p300 (N-15): sc-584 (C), β-catenin (H-102): sc-7199, β-catenin (C-18): sc-1496 and β-catenin (E-5): sc-7963 (D). Data kindly provided by M.G. Rosenfeld and reproduced wtih permission from Gold et al., Neuron 2003, 40: 1119-1131.
ChIP analysis of recruitment of CBP, p300, PCA and BRG1 to the IL-2α (CD25) promoter in vivo. Antibodies tested include CBP (A-22): sc-369, p300 (N-15): sc-584, PCAF (H-369): sc-8999, and Brg-1 (H-88): sc-10768. CD3ε and Oct-2 promoter regions were employed as positive and negative controls, respectively. DNA was isolated from Rag -/- thymocytes or Rag -/- thymocytes expressing TCRβ. Data kindly provided by J. Imbert and reproduced from Yeh, J-H., et al., Nucleic Acids Research, 2002, 30: 1944-1951, with permission from Oxford University Press. p300 (N-15): sc-584. Immunoperoxidase staining of formalin fixed, paraffin-embedded human esophagus tissue showing nuclear and cytoplasmic staining of squamous epithelial cells.
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