XPA Antibody (12F5): sc-56813

XPA Antibody (12F5) is a mouse monoclonal IgG2a kappa light chain antibody that detects XPA protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). XPA (12F5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. XPA protein plays a critical role in the nucleotide excision repair (NER) pathway, which is essential for repairing DNA damage caused by ultraviolet (UV) light. This function is particularly important as deficiencies in NER can lead to xeroderma pigmentosum, a genetic disorder that significantly increases the risk of skin cancer due to the accumulation of unrepaired DNA lesions. XPA coordinates the assembly of the preincision complex necessary for recognizing and processing damaged DNA, and exhibits a unique ability to bind to both single-stranded and double-stranded DNA. XPA′s structure allows formation of a homodimer in the absence of DNA, enhancing binding efficiency when DNA is present. This dimerization is crucial for the effective repair of bulky DNA adducts, as XPA′s activity is regulated in a concentration-dependent manner, ensuring cells can respond appropriately to varying levels of DNA damage. Anti-XPA antibody (12F5) serves as a valuable tool for studying the mechanisms of DNA repair and the implications of its dysfunction in human health.

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XPA Antibody (12F5) References:

1. Critical DNA damage recognition functions of XPC-hHR23B and XPA-RPA in nucleotide excision repair.  |  Thoma, BS. and Vasquez, KM. 2003. Mol Carcinog. 38: 1-13. PMID: 12949838
2. A new structural insight into XPA-DNA interactions.  |  Hilton, B., et al. 2014. Biosci Rep. 34: e00162. PMID: 25385088
3. Differential sensitivities of cellular XPA and PARP-1 to arsenite inhibition and zinc rescue.  |  Ding, X., et al. 2017. Toxicol Appl Pharmacol. 331: 108-115. PMID: 28552776
4. DNA repair protein XPA is differentially expressed in colorectal cancer and predicts better prognosis.  |  Feng, X., et al. 2018. Cancer Med. 7: 2339-2349. PMID: 29675892
5. XPA, XPC, and XPD Modulate Sensitivity in Gastric Cisplatin Resistance Cancer Cells.  |  Pajuelo-Lozano, N., et al. 2018. Front Pharmacol. 9: 1197. PMID: 30386247
6. Structural characterization of the redefined DNA-binding domain of human XPA.  |  Lian, FM., et al. 2019. Biochem Biophys Res Commun. 514: 985-990. PMID: 31092331
7. Increased levels of XPA might be the basis of cisplatin resistance in germ cell tumours.  |  Cierna, Z., et al. 2020. BMC Cancer. 20: 17. PMID: 31906898
8. Single molecule analysis reveals monomeric XPA bends DNA and undergoes episodic linear diffusion during damage search.  |  Beckwitt, EC., et al. 2020. Nat Commun. 11: 1356. PMID: 32170071
9. Potential UV-Protective Effect of Freestanding Biodegradable Nanosheet-Based Sunscreen Preparations in XPA-Deficient Mice.  |  Hatanaka, T., et al. 2022. Pharmaceutics. 14: PMID: 35214163
10. The DNA damage-recognition problem in human and other eukaryotic cells: the XPA damage binding protein.  |  Cleaver, JE. and States, JC. 1997. Biochem J. 328 (Pt 1): 1-12. PMID: 9359827

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-XPA Antibody (12F5) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-XPA Antibody (12F5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

XPA Antibody (12F5) Specifications

Alternate Product Name: XPA

Product Clone Name: 12F5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: XPA

Epitope Species: human

Product Reactivity/Detection: XPA

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide, 0.1% gelatin and 0.1% stabilizer protein

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for XPA Antibody (12F5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred XPA antibody is XPA Antibody (B-1): sc-28353.