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Pol II Antibody (8WG16): sc-56767

 |  Datasheet

(Based on data analysis)

  • Pol II Antibody (8WG16) is a mouse monoclonal IgG2a provided at 100 µg/ml
  • raised against purified RNA pol II of wheat germ origin
  • recommended for detection of the highly conserved heptapeptide repeat of the largest subunit of eukaryotic RNA pol II of mouse, human, Xenopus, C. elegans, yeast and wheat germ origin by WB, IP and IF; also reactive with additional species, including bovine
  • See 9 citations for Pol II Antibody (8WG16)
 

See additional RNA Polymerase Antibodies including RNA Polymerase, Pol I, Pol I/II/III RPB6, Pol I/II/III RPB8, Pol II, Pol III, RNA pol alpha, RNA pol beta, RNA pol sigma 70, Pol II RPB3, Pol II RPB4, Pol II RPB5, Pol II RPB6, RPA16, Pol III RPC32, Pol III RPC39, RPA40, Pol III RPC62, RPA135 and RPA194.

Also see chemical Pol II Inhibitors for functional analysis of cellular responses to Pol II.


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Recommended Support Products:
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 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
Pol II Antibody (8WG16) sc-56767 100 µg/ml $295
 CRISPR/Cas9 KO and HDR Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
Pol II CRISPR/Cas9 KO Plasmid (m) sc-422732 20 µg $345
Pol II HDR Plasmid (m) sc-422732-HDR 20 µg $385
Pol II CRISPR/Cas9 KO Plasmid (h) sc-400053 20 µg $345
Pol II HDR Plasmid (h) sc-400053-HDR 20 µg $385
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
Pol II siRNA (h) sc-36290 10 µM $258
Pol II siRNA (m) sc-36291 10 µM $258
Pol II (h)-PR sc-36290-PR 10 µM, 20 µl $23
Pol II (m)-PR sc-36291-PR 10 µM, 20 µl $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
Pol II shRNA Plasmid (h) sc-36290-SH 20 µg $520
Pol II shRNA Plasmid (m) sc-36291-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
Pol II shRNA (h) Lentiviral Particles sc-36290-V 200 µl $625
Pol II shRNA (m) Lentiviral Particles sc-36291-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
A-431 nuclear extract sc-2122 250 µg/0.05 ml $143
A-673 nuclear extract sc-2128 250 µg/0.05 ml $143
U-2 OS Cell Lysate sc-2295 500 µg/200 µl $104
HeLa nuclear extract sc-2120 250 µg/0.05 ml $143
NIH/3T3 Whole Cell Lysate sc-2210 500 µg/200 µl $104
HeLa Whole Cell Lysate sc-2200 500 µg/200 µl $104
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human POLR2A 5430 17p13.1 NM_000937 P24928
180660
Mouse Polr2a 20020 11 B3 P08775
N/A
 


RNA polymerase II (Pol II) is an enzyme that is composed of twelve subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1,840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats. Although Pol II function requires the cooperation of all twelve subunits, the largest subunit conveys Pol II catalytic activity and, together with the second largest subunit, forms the active center of the Pol II enzyme. Additionally, the large subunit participates in forming the DNA-binding domain of Pol II, a groove that is necessary for transcription of the DNA template. Without proper function of the large subunit, mRNA synthesis and subsequent transcription elongation cannot occur.

Pol II Antibody (8WG16) Product Citations
See how others have used Pol II Antibody (8WG16) and or Pol II Antibody (8WG16) conjugates.


9 total citations
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Pol II Antibody (8WG16) Data
Click on image to enlarge
Pol II (8WG16): sc-56767. Western blot analysis of Pol II expression in A-431 (A), A-673 (B), HeLa (C) and NIH/3T3 (D) nuclear extracts and HeLa whole cell lysate (E).
Western blot analysis of Pol II phosphorylation in untreated (A,D), calyculin A treated (B,E) and calyculin A and lambda protein phosphatase (sc-200312A) treated (C,F) HeLa whole cell lysates. Antibodies tested include p-Pol II (8A7): sc-13583 (A,B,C) and Pol II (8WG16): sc-56767 (D,E,F).
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