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home » Transcription Regulators » PML Antibody / PML Antibodies » PML (H-238): sc-5621

PML (H-238) Antibody: sc-5621

  |  Datasheet
  • rabbit polyclonal IgG, 200 µg/ml
  • epitope corresponding to amino acids 157-394 mapping to an internal region of PML of human origin
  • recommended for detection of all isoforms of PML of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including equine
  • TransCruz reagent for Gel Supershift and ChIP applications, sc-5621 X, 200 µg/0.1 ml
 
Additional PML Antibodies ...
 
Ordering InformationProduct Citations
Recommended Support Products:
(click button of application of choice)
WB   IP   IF   IHC(P)   Gel Shift   ChIP   siRNA  
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human PML 5371 15q24.1 NM_002675, NM_033238, NM_033239, NM_033240, NM_033244, NM_033246, NM_033247, NM_033249, NM_033250 P29590
 102578
Mouse Pml 18854 9 B Q60953
 N/A
 
Set Currency

 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
PML (H-238) sc-5621 200 µg/ml $279
PML (H-238) X sc-5621 X 200 µg/0.1 ml $279
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
PML siRNA (h) sc-36284 10 µM $258
PML siRNA (m) sc-36283 10 µM $258
PML (h)-PR sc-36284-PR 10 µM $23
PML (m)-PR sc-36283-PR 10 µM $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
PML shRNA Plasmid (h) sc-36284-SH 20 µg $520
PML shRNA Plasmid (m) sc-36283-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
PML shRNA (h) Lentiviral Particles sc-36284-V 200 µl $625
PML shRNA (m) Lentiviral Particles sc-36283-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
K-562 Whole Cell Lysate sc-2203 500 µg/200 µl $104
COLO 320DM Cell Lysate sc-2226 500 µg/200 µl $104
K-562 nuclear extract sc-2130 1000 µg $143
PML (m): 293T Lysate sc-122659 100µg/200µl $205
mouse cerebellum extract sc-2403 500 µg/200 µl $104

PML Background Information
The PML protein is a zinc finger transcription factor expressed as three major transcription products due to alternative splicing. The gene encoding human PML maps to chromosome 15q22. The t(15;17) (q22;q11.2-q12) chromosomal translocation of the retinoic acid receptor å (RARå) gene occurs in virtually all cases of acute promyelocytic leukemia and results in the expression of a PML/RARa chimeric protein. Myeloid precursor cells expressing the PML/RARå chimera fail to differentiate and exhibit an increased growth rate consequent to diminished apoptosis. PML/RARå transforms myeloid precursors by recruiting the nuclear co-repressor (N-CoR)-histone deacetylase complex that is essential to retinoic acid-dependent myeloid differentiation. PML/RARå also recruits DNA methyltransferases in order to induce gene hypermethylation and silencing, which ultimately facilitates leukemogenesis.

PML (H-238) Product Citations
See how others have used PML (H-238): sc-5621 antibody and or PML (H-238) antibody conjugates.


59 total citations
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PML (H-238)
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sc-5621
PML (H-238): sc-5621. Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear localization.
sc-5621
PML (H-238): sc-5621. Western blot analysis of human recombinant PML fusion protein.
sc-5621
PML (H-238): sc-5621. Immunoperoxidase staining of formalin fixed, paraffin-embedded human renal cancer tissue showing nuclear staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
sc-5621
PML (H-238): sc-5621. Western blot analysis of PML expression in non-transfected: sc-117752 (A) and truncated mouse PML transfected: sc-122659 (B) 293T whole cell lysates.
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