Cdc34 (H-81) Antibody sc-5616

santa cruz biotechnology, inc.

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Cdc34 (H-81) Antibody: sc-5616	\| Datasheet

rabbit polyclonal IgG, 200 µg/ml
epitope corresponding to amino acids 144-224 mapping near the C-terminus of Cdc34 of human origin
recommended for detection of Cdc34 of mouse and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine, bovine and porcine

Additional Cdc Antibodies ...

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WB IP IF IHC(P) siRNA

Species	Gene Name	Gene ID	Chromosome Location	Isoform (mRNA) Accession #	Protein Accession #	OMIM™ Number

Human	CDC34	997	19p13.3	NM_004359	P49427	116948
Mouse	Cdc34	216150	10 C1	NM_177613	Q8CFI2	N/A

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Ordering Information

Product Name	Catalog #	Unit	Price	Qty	Add	Favorites
Cdc34 (H-81)	sc-5616	200 µg/ml	$279

siRNA Gene Silencers (click product name for more information)

Product Name	Catalog #	Unit	Price
Cdc34 siRNA (h)	sc-35042	10 µM	$258
Cdc34 siRNA (m)	sc-37554	10 µM	$258
Cdc34 (h)-PR	sc-35042-PR	10 µM	$23
Cdc34 (m)-PR	sc-37554-PR	10 µM	$23

shRNA Plasmids (click product name for more information)

Product Name	Catalog #	Unit	Price	Qty	Add	Favorites
Cdc34 shRNA Plasmid (h)	sc-35042-SH	20 µg	$520
Cdc34 shRNA Plasmid (m)	sc-37554-SH	20 µg	$520

shRNA Lentiviral Particles (click product name for more information)

Product Name	Catalog #	Unit	Price	Qty	Add	Favorites
Cdc34 shRNA (h) Lentiviral Particles	sc-35042-V	200 µl	$625
Cdc34 shRNA (m) Lentiviral Particles	sc-37554-V	200 µl	$625

WB Positive Control Cell Lysates (click product name for more information)

Product Name	Catalog #	Unit	Price
K-562 nuclear extract	sc-2130	1000 µg	$143
A-431 nuclear extract	sc-2122	1000 µg	$143
HeLa nuclear extract	sc-2120	1000 µg	$143
HeLa + PMA nuclear extract	sc-2121	1000 µg	$143
HeLa Whole Cell Lysate	sc-2200	500 µg/200 µl	$104
MOLT-4 nuclear extract	sc-2151	1000 µg	$143
MCF7 nuclear extract	sc-2149	1000 µg	$143
Cdc34 (h): 293 Lysate	sc-113137	100µg/200µl	$205

Cdc34 Background Information
Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins. The cell division cycle (Cdc) genes are required at various points in the cell cycle. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. Cdc6 is the human homolog of Saccharomyces cerevisiae Cdc6, which is involved in the initiation of DNA replication. Cdc37 appears to facilitate Cdk4/cyclin D1 complex formation and has been shown to form a stable complex with Hsp90. Cdc34, Cdc27 and Cdc16 function as ubiquitin-conjugating enzymes. Cdc34 is thought to be the structural and functional homolog of Saccharomyces cerevisiae Cdc34, which is essential for the G1 to S phase transition. Cdc16 and Cdc27 are components of the APC (anaphase-promoting complex) which ubiquitinates cyclin B, resulting in cyclin B/Cdk complex degradation.

Cdc34 (H-81) Product Citations

See how others have used Cdc34 (H-81): sc-5616 antibody and or Cdc34 (H-81) antibody conjugates.

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Cdc34 (H-81)

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Cdc34 (H-81): sc-5616. Western blot analysis of Cdc34 expression in K-562 (A), A-431 (B), HeLa (C) and HeLa + PMA (D) nuclear extracts.

Cdc34 (H-81): sc-5616. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing nuclear and cytoplasmic staining.

Cdc34 siRNA (h): sc-35042. Western blot analysis of Cdc34 expression in non-transfected control (A) and Cdc34 siRNA transfected (B) HeLa cells. Blot probed with Cdc34 (H-81): sc-5616. GAPDH (FL-335): sc-25778 used as specificity and loading control.

Cdc34 (H-81): sc-5616. Immunoperoxidase staining of formalin fixed, paraffin-embedded human skin tissue showing nuclear and cytoplasmic staining of epidermal cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

Cdc34 (H-81): sc-5616. Western blot analysis of Cdc34 expression in non-transfected: sc-110760 (A) and human Cdc34 transfected: sc-113137 (B) 293 whole cell lysates.

Cdc34 (H-81): sc-5616. Western blot analysis of Cdc34 expression in non-transfected: sc-117752 (A) and mouse Cdc34 transfected: sc-119127 (B) 293T whole cell lysates.

Cdc34 (H-81): sc-5616. Western blot analysis of Cdc34 expression in K-562 nuclear extract.

Cdc34 (H-81): sc-5616. Western blot analysis of Cdc34 expression in non-transfected: sc-110760 (A) and human Cdc34 transfected: sc-174860 (B) 293 whole cell lysates.

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Cdc34 (H-81) Antibody: sc-5616