Bcl-2 Antibody (100/D5): sc-56015

Bcl-2 Antibody (100/D5) is a mouse monoclonal IgG1 antibody that detects Bcl-2 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Bcl-2 is a crucial regulator of apoptosis, playing a significant role in cell survival by preventing programmed cell death, which is vital for maintaining tissue homeostasis and protecting against diseases, including cancer. Bcl-2 is primarily located in the outer mitochondrial membrane, where anti-Bcl-2 antibody (100/D5) detects anti-apoptotic effects by stabilizing the mitochondrial membrane and preventing the release of pro-apoptotic factors like cytochrome c. This localization is essential because Bcl-2 effectively interacts with other members of the Bcl-2 family, such as Bax and Bak, thereby modulating their activity and influencing cell fate. Dysregulation of Bcl-2 expression or function can lead to various pathologies, including cancer, where normal apoptotic processes are disrupted, allowing for uncontrolled cell proliferation. Bcl-2 monoclonal antibody (100/D5) serves as an invaluable tool for researchers studying apoptosis and its implications in disease, providing insights into the mechanisms of cell survival and death.

The preferred Bcl-2 antibody is Bcl-2 Antibody (C-2): sc-7382.