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- mouse monoclonal IgG2a; 200 µg/ml
- raised against rat PCNA made in the protein A expression vector pR1T2T
- recommended for detection of PCNA p36 protein expressed at high level in proliferating cells by mouse, rat, human, insect and S. pombe origin by WB, IP, IF, IHC(P) and FCM
- marker for cells in early G1 and S phases of the cell cycle
- agarose conjugate for IP studies, sc-56 AC, 500 µg/0.25 ml agarose
- HRP conjugate, sc-56 HRP, 200 µg/ml
- fluorescein (sc-56 FITC) and rhodamine (sc-56 TRITC) conjugates for immunofluorescence, 200 µg/ml
- phycoerythrin (sc-56 PE), PerCP (sc-56 PerCP) and PerCP-Cy5.5 (sc-56 PCPC5) conjugates for FCM, 100 tests
- Alexa Fluor® 405 (sc-56 AF405), Alexa Fluor® 488 (sc-56 AF488) and Alexa Fluor® 647 (sc-56 AF647) conjugates for FCM or immunofluorescence; 100 µg/2 ml
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PCNA Background Information
The proliferating cell nuclear antigen (PCNA), a protein synthesized in early G1 and S phases of the cell cycle, functions in cell cycle progression, DNA replication and DNA repair. In early S phase, PCNA exhibits granular distribution and is absent from the nucleoli; however, in late S phase, it relocates to the nucleoli. PCNA exists in two basic forms: one involved in ongoing DNA replication, which localizes specifically to the nucleus, and a second, soluble form, not implicated in constant synthesis. Interestingly, the latter form degrades in the presence of organic solvents, rendering it undetectable by histological methods in tissues using organic fixatives, and thus also providing a method of visualizing only the synthesizing form.
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See how others have used PCNA (PC10): sc-56 antibody and or PCNA (PC10) antibody conjugates.
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PCNA (PC10)
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PCNA (PC10): sc-56. Immunofluorescence staining of methanol-fixed NIH/3T3 cells (A). Immunoperoxidase staining of formalin-fixed, paraffin-embedded human liver tumor showing strong nuclear staining of hepatocytes (B).
PCNA (PC10) PE: sc-56 PE. Intracellular FCM analysis of fixed and permeabilized MOLT-4 cells. Black line histogram represents the isotype control, normal mouse IgG 2a: sc-2867. PCNA (PC10) Alexa Fluor 488: sc-56 AF488. Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal mouse IgG 2a: sc-3891. PCNA (PC10) Alexa Fluor 647: sc-56 AF647. Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal mouse IgG 2a: sc-24637. PCNA (PC10): sc-56 Immunoperoxidase staining of formalin fixed, paraffin-embedded human urothelial cancer showing nuclear staining of tumor cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
PCNA (PC10): sc-56. Western blot analysis of PCNA p36 expression in A-431 (A) and NIH/3T3 (B) whole cell lysates.
PCNA (PC10): sc-56. Western blot analysis of PCNA expression in non-transfected 293T: sc-117752 (A), mouse PCNA transfected 293T: sc-127307 (B) and A-431 (C) whole cell lysates. PCNA (PC10): sc-56. Western blot analysis of PCNA expression in non-transfected: sc-117752 (A) and human PCNA transfected: sc-159920 (B) 293T whole cell lysates. PCNA (PC10): sc-56. Western blot analysis of PCNA expression in non-transfected: sc-117752 (A) and human PCNA transfected: sc-170834 (B) 293T whole cell lysates. PCNA (PC10) PerCP: sc-56 PerCP. Intracellular FCM analysis of fixed and permeabilized MOLT-4 cells. Black line histogram represents the isotype control, normal mouse IgG 2a: sc-45112. PCNA (PC10) AF647: sc-56 AF647. Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal mouse IgG 2a: sc-24637. PCNA (PC10) AF488: sc-56 AF488. Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal mouse IgG 2a: sc-3891. |
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