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- rabbit polyclonal IgG, 200 µg /ml
- epitope corresponding to amino acids 520-692 mapping near the C-terminus of Arnt 1 of human origin
- recommended for detection of Arnt 1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including equine, canine, bovine and porcine
- TransCruz reagent for Gel Supershift and ChIP applications, sc-5580 X, 200 µg/0.1 ml
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Arnt 1 Background Information AhR, Arnt 1, Arnt 2 and BMAL1 are members of a family of transcription factors that contain a basic helix-loop-helix motif and a common “PAS” motif. The aromatic (aryl) hydrocarbon receptor, AhR, is a ligand dependent transcription factor that interacts with specific DNA sequences termed xenobiotic responsive elements (XREs) to activate several genes including CYP1A1, glutathione S-transferase Ya subunit and DT-diaphorase. The Ah receptor nuclear translocator proteins (Arnt 1 or Arnt 2) are required for ligand-dependent nuclear translocation of the Ah receptor and are also necessary for Ah receptor binding to the XRE element. Arnt 1 (aryl hydrocarbon receptor nuclear translocator), also known as HIF1B, TANGO, bHLHe2, HIF1BETA, HIF-1beta ot ARNT, is a 789 amino acid nuclear protein that contains a basic helix-loop-helix (bHLH) domain, a PAC (PAS-associated C-terminal) domain and two PAS (PER-ARNT-SIM) domains.
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See how others have used Arnt 1 (H-172): sc-5580 antibody and or Arnt 1 (H-172) antibody conjugates.
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Arnt 1 (H-172)
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Western blot analysis of Arnt 1 expression in untreated (A,C) and CoCl 2 treated HeLa (B,D) whole cell lysates. Antibodies tested include Arnt 1 (N-19): sc-8077 (A,B) and Arnt 1 (H-172): sc-5580 (C,D).
Arnt 1 (H-172): sc-5580. Immunoperoxidase staining of formalin-fixed, paraffin-embedded mouse brain showing nuclear localization.
Arnt 1 (H-172): sc-5580. Western blot analysis of Arnt 1 expression in non-transfected: sc-117752 (A) and mouse Arnt 1 transfected: sc-118563 (B) 293T whole cell lysates.
Arnt 1 (H-172): sc-5580. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
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