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Arnt 1 (H-172) Antibody: sc-5580

 |  Datasheet
  • rabbit polyclonal IgG, 200 µg /ml
  • epitope corresponding to amino acids 520-692 mapping near the C-terminus of Arnt 1 of human origin
  • recommended for detection of Arnt 1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including equine, canine, bovine and porcine
  • TransCruz reagent for Gel Supershift and ChIP applications, sc-5580 X, 200 µg/0.1 ml
 
Additional Arnt Antibodies ...
 
Ordering InformationProduct Citations
Recommended Support Products:
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WB   IP   IF   IHC(P)   Gel Shift   ChIP   siRNA  
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human ARNT 405 1q21.2 NM_001668, NM_178426, NM_178427 P27540
126110
Mouse Arnt 11863 3 F2.1 P53762
N/A
 
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 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
Arnt 1 (H-172) sc-5580 200 µg/ml $279
Arnt 1 (H-172) X sc-5580 X 200 µg/0.1 ml $279
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
Arnt 1 siRNA (h) sc-29733 10 µM $258
Arnt 1 siRNA (m) sc-29734 10 µM $258
Arnt 1 siRNA (r) sc-156041 10 µM $258
Arnt 1 (h)-PR sc-29733-PR 10 µM $23
Arnt 1 (m)-PR sc-29734-PR 10 µM $23
Arnt 1 (r)-PR sc-156041-PR 10 µM, 20 µl $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
Arnt 1 shRNA Plasmid (h) sc-29733-SH 20 µg $520
Arnt 1 shRNA Plasmid (m) sc-29734-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
Arnt 1 shRNA (h) Lentiviral Particles sc-29733-V 200 µl $625
Arnt 1 shRNA (m) Lentiviral Particles sc-29734-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
HeLa Whole Cell Lysate sc-2200 500 µg/200 µl $104
HeLa + CoCl2 Cell Lysate sc-24679 500 µg/200 µl $104
K-562 nuclear extract sc-2130 1000 µg $143
K-562 Whole Cell Lysate sc-2203 500 µg/200 µl $104
WEHI-231 Whole Cell Lysate sc-2213 500 µg/200 µl $104
RAW 264.7 Whole Cell Lysate sc-2211 500 µg/200 µl $104
NIH/3T3 nuclear extract sc-2138 1000 µg $143

Arnt 1 Background Information
AhR, Arnt 1, Arnt 2 and BMAL1 are members of a family of transcription factors that contain a basic helix-loop-helix motif and a common “PAS” motif. The aromatic (aryl) hydrocarbon receptor, AhR, is a ligand dependent transcription factor that interacts with specific DNA sequences termed xenobiotic responsive elements (XREs) to activate several genes including CYP1A1, glutathione S-transferase Ya subunit and DT-diaphorase. The Ah receptor nuclear translocator proteins (Arnt 1 or Arnt 2) are required for ligand-dependent nuclear translocation of the Ah receptor and are also necessary for Ah receptor binding to the XRE element. Arnt 1 (aryl hydrocarbon receptor nuclear translocator), also known as HIF1B, TANGO, bHLHe2, HIF1BETA, HIF-1beta ot ARNT, is a 789 amino acid nuclear protein that contains a basic helix-loop-helix (bHLH) domain, a PAC (PAS-associated C-terminal) domain and two PAS (PER-ARNT-SIM) domains.

Arnt 1 (H-172) Product Citations
See how others have used Arnt 1 (H-172): sc-5580 antibody and or Arnt 1 (H-172) antibody conjugates.


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Arnt 1 (H-172)
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Western blot analysis of Arnt 1 expression in untreated (A,C) and CoCl2 treated HeLa (B,D) whole cell lysates. Antibodies tested include Arnt 1 (N-19): sc-8077 (A,B) and Arnt 1 (H-172): sc-5580 (C,D).
Arnt 1 (H-172): sc-5580. Immunoperoxidase staining of formalin-fixed, paraffin-embedded mouse brain showing nuclear localization.
Arnt 1 (H-172): sc-5580. Western blot analysis of Arnt 1 expression in non-transfected: sc-117752 (A) and mouse Arnt 1 transfected: sc-118563 (B) 293T whole cell lysates.
Arnt 1 (H-172): sc-5580. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
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