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NF-1 Antibody (H-300): sc-5567

 |  Datasheet

(Based on data analysis)

  • NF-1 Antibody (H-300) is a rabbit polyclonal IgG provided at 200 µg/ml
  • epitope corresponding to amino acids 1-300 mapping at the N-terminus of NF-1 of human origin
  • recommended for detection of all NF-1 isoforms of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine, bovine and porcine
  • TransCruz reagent for Gel Supershift and ChIP applications, sc-5567 X, 200 µg/0.1 ml
  • See 28 citations for NF-1 Antibody (H-300)
 

See additional NF-1 Antibodies including NF-1, NF-1, NF-1A and NF-1C.

Ordering InformationProduct CitationsGene Info
Recommended Support Products:
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WB   IP   IF   IHC(P)   Gel Shift   ChIP  
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 Ordering Information
Product NameCatalog #UnitPriceQtyAdd 
NF-1 Antibody (H-300) sc-5567 200 µg/ml $279
NF-1 Antibody (H-300) X sc-5567 X 200 µg/0.1 ml $279
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
NIH/3T3 + PMA nuclear extract sc-2125 250 µg/0.05 ml $143
HeLa + PMA nuclear extract sc-2121 250 µg/0.05 ml $143
NF-1 (m2): 293T Lysate sc-122030 100 µg/200 µl $205
HeLa + PMA Cell Lysate sc-2258 500 µg/200 µl $104
NF-1 (h5): 293T Lysate sc-159124 100 µg/200 µl $205
NIH/3T3 nuclear extract sc-2138 250 µg/0.05 ml $143
NF-1 (h): 293 Lysate sc-110509 100 µg/200 µl $205
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human NFIC 4782 19p13.3 NM_005597, NM_205843 P08651
 600729
Mouse Nfic 18029 10 C1 P70255
 N/A
 

NF-1, also designated CTF, consists of a family of CCAAT box binding proteins that stimulate DNA replication and activate transcription. Analysis of human NF-1 messenger RNA has revealed two forms of the NF-1 protein arising from an alternate splicing of a single NF-1 gene. NF-1 binds its consensus DNA element as a homodimer via an amino-terminal DNA binding domain, and activates transcription through a putatively novel, proline-rich, carboxy terminal transactivation domain. The NF-1 protein has been shown to recognize and bind the adenovirus type 2 promoter and activate transcription of herpes simplex virus thymidine kinase genes. The NF-1 consensus element has been found in the upstream promoter region of myriad eukaryotic genes, including that of Ha-Ras, å-globin, HSP 70, GRP 78, Histone H1, myelin basic protein and in the Xenopus laevis vitellogenin gene promoter.

NF-1 Antibody (H-300) Product Citations
See how others have used NF-1 Antibody (H-300) and or NF-1 Antibody (H-300) conjugates.


28 total citations
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NF-1 Antibody (H-300) Data
Click on image to enlarge
sc-5567
NF-1 (H-300): sc-5567. Western blot analysis of NF-1 expression in non-transfected: sc-117752 (A) and mouse NF-1 transfected: sc-122030 (B) 293T whole cell lysates.
sc-5567
NF-1 (H-300): sc-5567. Western blot analysis of NF-1 expression in non-transfected: sc-110760 (A) and human NF-1 transfected: sc-110509 (B) 293 whole cell lysates.
sc-5567
NF-1 (H-300): sc-5567. Western blot analysis of NF-1 expression in untreated (A) and PMA-treated (B) HeLa whole cell lysates.
sc-5567
NF-1 (H-300): sc-5567. Western blot analysis of NF-1 expression in non-transfected: sc-117752 (A) and human NF-1 transfected: sc-159124 (B) 293T whole cell lysates.
sc-5567
NF-1 (H-300): sc-5567. Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing nuclear and cytoplasmic staining of glandular cells.
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