NOS1 Antibody (H-7): sc-55521

NOS1 Antibody (H-7) is a mouse monoclonal IgG2b kappa light chain antibody engineered to detect neuronal nitric oxide synthase (nNOS), also known as NOS1 or ncNOS. NOS1 monoclonal antibody (H-7) is raised against amino acids 2-300 of the human NOS1 protein, ensuring effective recognition of NOS1 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Neuronal nitric oxide synthase is a critical enzyme responsible for the production of nitric oxide (NO), a versatile signaling molecule involved in processes like neurotransmission, synaptic plasticity, and neuronal communication. The structure of NOS1 comprises an N-terminal oxygenase domain and a C-terminal reductase domain, which are essential for enzymatic activity and require homodimerization for proper function. This structural organization allows NOS1 to facilitate the conversion of L-arginine to NO, a reaction dependent on multiple cofactors including NADPH, FAD, FMN, calmodulin, tetrahydrobiopterin, and heme. Understanding the intricate structure of NOS1 is vital as this knowledge underpins the enzyme′s role in regulating precise NO production within neurons, thereby influencing various physiological and pathological processes. NOS1′s structural domains also differentiate NOS1 from other nitric oxide synthase isoforms, such as inducible NOS2 and endothelial NOS3, each with unique functional implications. Anti-NOS1 antibody (H-7) is available in both non-conjugated form and a variety of conjugated forms, including horseradish peroxidase (HRP), fluorescein isothiocyanate (FITC), and Alexa Fluor® conjugates, providing versatility for diverse experimental setups. By enabling detailed analysis of NOS1′s structural and functional properties, NOS1 (H-7) antibody supports advanced research into NO-mediated neuronal signaling and its impact on health and disease, ultimately aiding in the development of targeted therapeutic strategies.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NOS1 Antibody (H-7) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-NOS1 Antibody (H-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NOS1 Antibody (H-7) Specifications

Alternate Product Name: NOS1

Product Clone Name: H-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 2-300

Epitope Target: NOS1

Epitope Species: human

Product Reactivity/Detection: NOS1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 12 citations in various scientific publications

Available Conjugates/Forms for NOS1 Antibody (H-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred NOS1 antibody is NOS1 Antibody (A-11): sc-5302.