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- goat polyclonal IgG, 200µg/ml
- epitope mapping within an internal region of Edc3 of human origin
- recommended for detection of Edc3 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine, canine, porcine and avian
- blocking peptide, sc-55081 P
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Ordering Information
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Edc3 Background Information
The major eukaryotic mRNA decay pathway occurs through deadenylation, decapping, and 5' to 3' degradation of the mRNA. Decapping is a critical control point in this decay pathway. During the process of mRNA degradation, Edc3 has been found to play a role in mRNA decapping. As part of the mRNA degradation process, Edc3 becomes part of a complex that also contains hDcp1a, hDcp2a, RCK and Edc4/HEDLS. Within this complex, Edc3 directly interacts with DCP1A and DDX6. Edc3, enhancer of mRNA-decapping protein 3, is a 508 amino acid protein that maps to human gene EDC3. Edc3 is a member of the EDC3 family and contains one YjeF N-terminal domain. Edc3 is localized to the cytoplasm and is found primarily in the processing bodies (PBs) of the cell. Evidence indicates Edc3 also interacts with TTP (zinc finger protein 36), a candidate gene for obesity-related metabolic complications. |
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Edc3 (G-16)
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Edc3 (G-16): sc-55081. Western blot analysis of Edc3 expression in HeLa nuclear extract (A) and HeLa (B), 3T3-L1 (C), PC-3 (D) and DU 145 (E) whole cell lysates.
Edc3 (G-16): sc-55081. Western blot analysis of Edc3 expression in non-transfected: sc-110760 (A) and human Edc3 transfected: sc-111192 (B) 293 whole cell lysates. Edc3 (G-16): sc-55081. Western blot analysis of Edc3 expression in non-transfected: sc-117752 (A) and mouse Edc3 transfected: sc-119916 (B) 293T whole cell lysates. Edc3 (G-16): sc-55081. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasm and mRNA processing body (p-body) localization.
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