Date published: 2025-9-10
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western blotting image from recommended Estrogen Receptor alpha (F-10) monoclonal antibody replacement. Image 369721
This polyclonal antibody has been discontinued. See our recommended Estrogen Receptor alpha (F-10) monoclonal antibody (sample data shown).
western blotting image from recommended Estrogen Receptor alpha (F-10) monoclonal antibody replacement. Image 369721
This polyclonal antibody has been discontinued. See our recommended Estrogen Receptor alpha (F-10) monoclonal antibody (sample data shown).
This polyclonal antibody has been discontinued. See our recommended Estrogen Receptor alpha (F-10) monoclonal antibody (sample data shown).

ERα Antibody (HC-20): sc-543

4.5(22)
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  • ERα Antibody (HC-20) is a rabbit polyclonal IgG; 100 µg/ml
  • epitope mapping at the C-terminus of ERα of human origin
  • Discontinued polyclonal antibody

SEE ALSO...

Additional Estrogen Receptor Antibodies including Estrogen Receptor alpha, ER β, ERR α, ERR β and ERR γ

Ordering Information

Estrogen Receptor alpha (F-10): sc-8002 [ Recommended monoclonal replacement for ERα (HC-20) ]

Product NameCatalog #UNITPriceQtyFAVORITES

Estrogen Receptor alpha Antibody (F-10)

sc-8002
200 µg/ml
$316.00

Estrogen Receptor alpha Antibody (F-10): m-IgG Fc BP-HRP Bundle

sc-528195
200 µg Ab; 10 µg BP
$354.00

Estrogen Receptor alpha Antibody (F-10): m-IgG2a BP-HRP Bundle

sc-547044
200 µg Ab; 10 µg BP
$354.00

Estrogen Receptor alpha Antibody (F-10): m-IgGκ BP-HRP Bundle

sc-520517
200 µg Ab, 40 µg BP
$354.00

Estrogen Receptor alpha Antibody (F-10) X

sc-8002 X
200 µg/0.1 ml
$316.00

Estrogen Receptor alpha Antibody (F-10) AC

sc-8002 AC
500 µg/ml, 25% agarose
$416.00

Estrogen Receptor alpha Antibody (F-10) Alexa Fluor® 546

sc-8002 AF546
200 µg/ml
$357.00

Estrogen Receptor alpha Antibody (F-10) Alexa Fluor® 594

sc-8002 AF594
200 µg/ml
$357.00

Estrogen Receptor alpha Antibody (F-10) Alexa Fluor® 680

sc-8002 AF680
200 µg/ml
$357.00

Estrogen Receptor alpha Antibody (F-10) Alexa Fluor® 790

sc-8002 AF790
200 µg/ml
$357.00

Estrogen Receptor alpha (F-10) Neutralizing Peptide

sc-8002 P
100 µg/0.5 ml
$68.00

For a manuscript under revision I urgently need accurate information about the epitope used to generate the HC-20 anti-ERalpha polyclonal antibody. I have a text document stating that it is the last 50 amino acids, but find nothing online?

Asked by: HelenaP
Thank you for your question. Please contact our technical service team directly for further information on this product.
Answered by: Tech Support Europe
Date published: 2022-12-02

Hi I was using sc-543x for my chip experiments. I have few experiments left to do with the same antibody. why have been this antibody discontinued? Thanks

Asked by: prati
Thank you for your question. Yes, our rabbit polyclonal antibody, ERα (HC-20): sc-543, has been discontinued and is no longer available. Our highly recommended replacement monoclonal antibodies for ERα have also been validated for ChIP. Both clones, ERα (F-10): sc-8002 and ERα (D-12): sc-8005, have been cited in numerous publications for use in Chromatin Immunoprecipitation (ChIP) applications. Please contact our Technical Service Department if you would like to request a free 10 µg sample of these antibodies to test in your application. Please call us toll-free at (800) 457-3801 option 2, e-mail <scbt@scbt.com>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2021-11-04
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Rated 5 out of 5 by from WB ERWb for Estrogen receptor in MCF7 cells, 50ug of whole protein extract were used
Date published: 2019-02-13
Rated 4 out of 5 by from ER-alpha antibody western blotIt is a good antibody for Er-alpha detection by Western blot . We used it in a 1:1000 dilution in human placenta samples and the results can be observed in this paper. It is an antibody of specific recognition.
Date published: 2018-05-17
Rated 3 out of 5 by from Used to detect ER alpha protein levelsThis antibody is very useful for detection of ER alpha protein levels in animal brain tissue. In particular, we used this antibody to detect protein levels in mytosol in hippocampus and prefrontal cortex of rats. In general, we are satisfied with the obtained results from our samples. Dilutions of Ab, up to 1:1000.
Date published: 2017-11-23
Rated 5 out of 5 by from Good for WBI tested this antibody for western blot using MCF7 total protein. As seen in photo, I got a specific band of ERalpha.
Date published: 2017-01-10
Rated 5 out of 5 by from Published WB and ChIP data on HMLE and MCF10APublished WB and ChIP data on HMLE and MCF10A cells and on MEF
Date published: 2016-06-24
Rated 5 out of 5 by from It is very clearn for western blotIt is very clearn for western blot
Date published: 2015-10-20
Rated 5 out of 5 by from I have used this antibody to do CHIP assayI have used this antibody to do CHIP assay as many paper used. To be honest, it works quite well. What s more, it is much cheaper than the similar products of other company.
Date published: 2015-10-15
Rated 5 out of 5 by from I used this in WB in mouse cells and works greatI used this in WB in mouse cells and works great, correct band seen at 1:500 dilution.
Date published: 2015-10-07
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ERα Antibody (HC-20) is rated 4.5 out of 5 by 22.
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