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ERα Antibody (HC-20): sc-543

 |  Datasheet

(Based on data analysis)

  • ERα Antibody (HC-20) is a rabbit polyclonal IgG provided at 200 µg/ml
  • epitope mapping at the C-terminus of ERα of human origin
  • recommended for detection of estrogen receptor α of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including bovine
  • blocking peptide, sc-543 P
  • TransCruz reagent for Gel Supershift and ChIP applications, sc-543 X, 200 µg/0.1 ml
  • See 197 citations for ERα Antibody (HC-20)
 

See additional Estrogen Receptor Antibodies including Estrogen Receptor, ER alpha, ER beta, ERR alpha, ERR beta and ERR gamma.

Also see ER alpha Inhibitors or ER alpha Activators for functional analysis of cellular responses to ER alpha.


Ordering InformationProduct CitationsGene Info
Recommended Support Products:
(click button of application of choice)
WB   IP   IF   IHC(P)   Gel Shift   ChIP   siRNA  
Set Currency

 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
ERα Antibody (HC-20) sc-543 200 µg/ml $279
ERα (HC-20) P sc-543 P
(peptide)
100 µg/0.5 ml $61
ERα Antibody (HC-20) X sc-543 X 200 µg/0.1 ml $279
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
ERα siRNA (h) sc-29305 10 µM $258
ERα siRNA (m) sc-29306 10 µM $258
ERα (h)-PR sc-29305-PR 10 µM, 20 µl $23
ERα (m)-PR sc-29306-PR 10 µM, 20 µl $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
ERα shRNA Plasmid (h) sc-29305-SH 20 µg $520
ERα shRNA Plasmid (m) sc-29306-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
ERα shRNA (h) Lentiviral Particles sc-29305-V 200 µl $625
ERα shRNA (m) Lentiviral Particles sc-29306-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
MCF7 Whole Cell Lysate sc-2206 500 µg/200 µl $104
MCF7 nuclear extract sc-2149 250 µg/0.05 ml $143
T-47D Cell Lysate sc-2293 500 µg/200 µl $104
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human ESR1 2099 6q25.1 NM_000125 P03372
608446
Mouse Esr1 13982 10 A1 P19785
N/A
 


Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ERå and ER∫, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus , though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ERå and ER∫ have been shown to be differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ERå and ER∫ may be regulated by distinct mechanisms even though they share many functional characteristics.

ERα Antibody (HC-20) Product Citations
See how others have used ERα Antibody (HC-20) and or ERα Antibody (HC-20) conjugates.


197 total citations
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ERα Antibody (HC-20) Data
Click on image to enlarge
ERα (HC-20): sc-543. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear localization.
ERα (HC-20): sc-543. Western blot analysis of human recombinant ERα (A), ERα expression in MCF7 nuclear extract (B) and T-47D whole cell lysate (C). Note amino terminal truncated form of ERα.
ERa (HC-20): sc-543. Immunofluorescence staining of methanol-fixed T-47D cells showing nuclear localization.
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