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ERα Antibody (HC-20): sc-543


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  • ERα Antibody (HC-20) is a rabbit polyclonal IgG provided at 100 µg/ml (Note concentration change)
  • epitope mapping at the C-terminus of ERα of human origin
  • recommended for detection of estrogen receptor α of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including bovine
  • blocking peptide, sc-543 P
  • TransCruz reagent for Gel Supershift and ChIP applications, sc-543 X, 100 µg/0.1 ml (Note concentration change)
  • See 231 citations for ERα Antibody (HC-20)

Monos, satisfaction guaranteed

ERα (HC-20) will be discontinued December 31, 2016; to receive a 10 µg sample of ERα (C-3): sc-514857, our highly recommended and much superior monoclonal antibody alternative to this product, please contact Technical Service.


See additional Estrogen Receptor Antibodies including Estrogen Receptor, ER α, ER β, ERR α, ERR β and ERR γ.

Also see chemical ER alpha Inhibitors or chemical ER alpha Activators for functional analysis of cellular responses to ER α.

Ordering InformationProduct CitationsGene Info
Recommended Support Products:
(click button of application of choice)
WB   IP   IF   IHC(P)   Gel Shift   ChIP   siRNA  
Set Currency

 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
ERα (HC-20) P sc-543 P
100 µg/0.5 ml $61
ERα Antibody (HC-20) sc-543 100 µg/ml $279
ERα Antibody (HC-20) X sc-543 X 100 µg/0.1 ml $279
 CRISPR KO, HDR and Nickase Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
ERα CRISPR/Cas9 KO Plasmid (h) sc-400011 20 µg $345
ERα HDR Plasmid (h) sc-400011-HDR 20 µg $385
ERα Double Nickase Plasmid (h) sc-400011-NIC 20 µg $355
ERα Double Nickase Plasmid (h2) sc-400011-NIC-2 20 µg $355
 CRISPR Activation Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
ERα CRISPR Activation Plasmid (h) sc-400011-ACT 20 µg $345
ERα CRISPR Activation Plasmid (h2) sc-400011-ACT-2 20 µg $345
ERα Lentiviral Activation Particles (h) sc-400011-LAC 200 µl $395
ERα Lentiviral Activation Particles (h2) sc-400011-LAC-2 200 µl $395
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
ERα siRNA (h) sc-29305 10 µM $258
ERα siRNA (m) sc-29306 10 µM $258
ERα (h)-PR sc-29305-PR 10 µM, 20 µl $23
ERα (m)-PR sc-29306-PR 10 µM, 20 µl $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
ERα shRNA Plasmid (h) sc-29305-SH 20 µg $520
ERα shRNA Plasmid (m) sc-29306-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
ERα shRNA (h) Lentiviral Particles sc-29305-V 200 µl $625
ERα shRNA (m) Lentiviral Particles sc-29306-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
MCF7 Whole Cell Lysate sc-2206 500 µg/200 µl $104
MCF7 nuclear extract sc-2149 250 µg/0.05 ml $143
T-47D Cell Lysate sc-2293 500 µg/200 µl $104
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human ESR1 2099 6q25.1 NM_000125 P03372
Mouse Esr1 13982 10 A1 P19785

Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ERα and ERβ, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus , though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ERα and ERβ have been shown to be differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ERα and ERβ may be regulated by distinct mechanisms even though they share many functional characteristics.

ERα Antibody (HC-20) Product Reviews

18 total reviews.
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ERα Antibody (HC-20) Product Citations
See how others have used ERα Antibody (HC-20) and or ERα Antibody (HC-20) conjugates.

231 total citations
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ERα Antibody (HC-20) Data
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ERα (HC-20): sc-543. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear localization.
ERα (HC-20): sc-543. Western blot analysis of human recombinant ERα (A), ERα expression in MCF7 nuclear extract (B) and T-47D whole cell lysate (C). Note amino terminal truncated form of ERα.
ERa (HC-20): sc-543. Immunofluorescence staining of methanol-fixed T-47D cells showing nuclear localization.