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- goat polyclonal IgG, 200µg/ml
- epitope mapping within an internal region of NIRF of human origin
- recommended for detection of NIRF of mouse, rat and human origin by WB, IP, IF and ELISA
- TransCruz reagent for Gel Supershift and ChIP applications, sc-54252 X, 200 µg/0.1 ml
- blocking peptide, sc-54252 P
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Ordering Information
Recommended Support Products:
(click button of application of choice)
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| Species |
Gene Name |
Gene ID |
Chromosome Location |
Isoform (mRNA) Accession # |
Protein Accession # |
OMIM™ Number |
| Human |
UHRF2 |
115426 |
9p24.1 |
NM_152896 |
Q96PU4
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n/a |
| Mouse |
Uhrf2 |
109113 |
19 C1 |
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Q7TMI3
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N/A |
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NIRF Background Information
NIRF (Np95/ICBP90-like RING finger protein), also known as E3 ubiquitin-protein ligase UHRF2, Nuclear zinc finger protein Np97 or RING finger protein 107, is a nuclear protein involved in cell cycle regulation. NIRF contains a PHD finger, two RING fingers, a ubiquitin-like domain and a YDG/SRA domain. It shares high structural homology with UHRF1 (also called ICBP90 in humans and Np95 in mice), however, in contrast to UHRF1, NIRF acts as a negative regulator of cell proliferation. It associates with the Cdk2-cyclin complex in its dephosphorylated form and induces G1 arrest. NIRF plays an important role in the regulation of the G1/S transition by blocking cell entry into the S-phase. While associated with Cdk2, NIRF becomes phosphorylated. NIRF can also act as a ubiquitin ligase and it ubiquitinates PCNP. In addition, NIRF can recruit and bind HDAC1 via its SRA domain. The overexpression of NIRF results in an increase of G1 phase cells. |
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NIRF (T-18)
Click on image to enlarge
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NIRF (T-18): sc-54252. Western blot analysis of NIRF expression in non-transfected: sc-110760 (A) and human NIRF transfected: sc-170276 (B) 293 whole cell lysates. |
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