epitope mapping within an internal region of HCP1 of mouse origin
recommended for detection of heme carrier protein 1 of mouse and rat origin by WB, IF and ELISA; also reactive with additional species, including canine
HCP1 Background Information HCP1 (Heme carrier protein 1), also known as proton-coupled folate transporter (PCFT), is a multi-pass transmembrane protein that is expressed in the small intestine. It is predominantly found in the duodenum and the jejunum localizing to the apical brush border. HCP1 is required for normal folate absorption in the intestine and is associated with folate homeostasis. HCP1 mediates the transport of folate and functions most optimally at a low extracellular pH of approximately 5.5. HCP1 functions independently of Na+ and is insensitive to membrane potential. It exhibits high affinity for folic acid and low affinity for the PT523 antifolate. HCP1 is post-translationally regulated by iron levels in the duodenum. During iron deficiency, HCP1 localizes to the apical membrane; however, iron excess causes HCP1 to localize in the cytoplasm. Sulfasalazine is a potent inhibitor of HCP1. Mutations in the gene encoding HCP1 can result in the autosomal recessive disorder hereditary folate malabsorption (HFM).
