Cdc2 p34 Antibody (17): sc-54

Cdc2 p34 Antibody (17) is a mouse monoclonal IgG2a kappa light chain antibody that detects Cdc2 p34 protein of mouse, rat, human, and Xenopus laevis origin by western blotting, immunoprecipitation, immunofluorescence, and immunohistochemistry. Cdc2 p34 (17) antibody is offered in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. Cdc2 p34, also known as cyclin-dependent kinase 1 (CDK1), plays a crucial role in regulating the cell cycle, particularly in the transition from the G2 phase to mitosis. This regulation is vital for proper cell division and ensuring that cells replicate their DNA accurately before mitosis, thereby preventing genomic instability and potential tumorigenesis. In vertebrates, Cdc2 p34 interacts with various cyclins, which are essential for activation and function, highlighting the importance in cell cycle control. Additionally, the presence of other cyclin-dependent kinases, such as Cdk2, which also binds to cyclins, underscores the complexity of cell cycle regulation in vertebrates. Cdc2 p34 monoclonal antibody (17) detection capabilities make this reagent an invaluable tool for researchers studying cell cycle dynamics and related processes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Cdc2 p34 Antibody (17) References:

1. Cyclin A is required at two points in the human cell cycle.  |  Pagano, M., et al. 1992. EMBO J. 11: 961-71. PMID: 1312467
2. CDK2 encodes a 33-kDa cyclin A-associated protein kinase and is expressed before CDC2 in the cell cycle.  |  Elledge, SJ., et al. 1992. Proc Natl Acad Sci U S A. 89: 2907-11. PMID: 1372993
3. Distinct forms of human CDC2 identified by novel monoclonal antibodies.  |  Lukás, J., et al. 1992. Eur J Biochem. 207: 169-76. PMID: 1628647
4. Human D-type cyclin.  |  Xiong, Y., et al. 1991. Cell. 65: 691-9. PMID: 1827756
5. Cyclins and their partners during Xenopus oocyte maturation.  |  Kobayashi, H., et al. 1991. Cold Spring Harb Symp Quant Biol. 56: 437-47. PMID: 1840257
6. Reversible tyrosine phosphorylation of cdc2: dephosphorylation accompanies activation during entry into mitosis.  |  Morla, AO., et al. 1989. Cell. 58: 193-203. PMID: 2473839
7. The cdc2 kinase is a nuclear protein that is essential for mitosis in mammalian cells.  |  Riabowol, K., et al. 1989. Cell. 57: 393-401. PMID: 2541912
8. Isolation of a human cyclin cDNA: evidence for cyclin mRNA and protein regulation in the cell cycle and for interaction with p34cdc2.  |  Pines, J. and Hunter, T. 1989. Cell. 58: 833-46. PMID: 2570636
9. Cip1 blocks the initiation of DNA replication in Xenopus extracts by inhibition of cyclin-dependent kinases.  |  Strausfeld, UP., et al. 1994. Curr Biol. 4: 876-83. PMID: 7850420
10. G2 delay induced by nitrogen mustard in human cells affects cyclin A/cdk2 and cyclin B1/cdc2-kinase complexes differently.  |  O'Connor, PM., et al. 1993. J Biol Chem. 268: 8298-308. PMID: 8463339
11. Expression and subcellular localization of CDK2 and cdc2 kinases and their common partner cyclin A in thyroid epithelial cells: comparison of cyclic AMP-dependent and -independent cell cycles.  |  Baptist, M., et al. 1996. J Cell Physiol. 166: 256-73. PMID: 8591985
12. Both cyclin A and cyclin E have S-phase promoting (SPF) activity in Xenopus egg extracts.  |  Strausfeld, UP., et al. 1996. J Cell Sci. 109 (Pt 6): 1555-63. PMID: 8799842

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Cdc2 p34 Antibody (17) has 746 citations in a variety of scientific publications.

Primary antibodies like Anti-Cdc2 p34 Antibody (17) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Cdc2 p34 Antibody (17) Specifications

Alternate Product Name: Cdc2 p34

Product Clone Name: 17

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 224-230

Epitope Target: Cdc2 p34

Epitope Species: human

Product Reactivity/Detection: Cdc2 p34

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: Xenopus

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 746 citations in various scientific publications

Available Conjugates/Forms for Cdc2 p34 Antibody (17): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, TRITC: Rhodamine