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- goat polyclonal IgG, 200 µg/ml
- epitope mapping within an internal region of GFP of Aequorea victoria origin
- recommended for detection of GFP and GFP mutant fusion proteins by WB, IP, IF and ELISA
- blocking peptide, sc-5385 P
- agarose conjugate for IP studies, sc-5385 AC, 500 µg/0.25 ml agarose
- fluorescein (sc-5385 FITC) and rhodamine (sc-5385 TRITC) conjugates for immunofluorescence, 200 µg/ml
- HRP conjugate, sc-5385 HRP, 200 µg/ml
- Alexa Fluor® 405 (sc-5385 AF405), Alexa Fluor® 488 (sc-5385 AF488) and Alexa Fluor® 647 (sc-5385 AF647) conjugates for immunofluorescence; 100 µg/2 ml
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GFP Background Information
The green fluorescent protein (GFP) was originally identified as a protein involved in the bioluminescence of the jellyfish Aequorea victoria. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors, making GFP a useful tool for monitoring gene expression and protein localization in vivo. Several GFP mutants have been developed, including EGFP, which fluoresce more intensely than the wildtype GFP and have shifted excitation maxima, making them useful for FACS and fluorescence microscopy as well as double-labeling applications. GFP is widely used in expression vectors as a fusion protein tag, allowing expression and monitoring of heterologous proteins fused to GFP.
| GFP (I-16) Product Citations |
See how others have used GFP (I-16): sc-5385 antibody and or GFP (I-16) antibody conjugates.
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GFP (I-16)
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GFP (I-16): sc-5385. Western blot analysis of GFP expression in COS (A) and GFP transfected COS (B) whole cell lysates.
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