epitope mapping near the C-terminus of TRFP of human origin
recommended for detection of TRFP of mouse, rat and human origin by ELISA; also reactive with additional species, including equine, canine, bovine and porcine
blocking peptide, sc-5380 P
TransCruz reagent for Gel Supershift and ChIP applications, sc-5380 X, 200 µg/0.1 ml
TRFP Background Information In mammalian cells, transcription is regulated in part by high molecular weight coactivating complexes that mediate signals between transcriptional activators and RNA polymerase (1). These complexes include SMCC (SRB and MED protein cofactor complex), which consists of various subunits that share homology with several components of the yeast transcriptional mediator complexes (2,3). SMCC associates with the RNAPII (RNA polymerase II) holoenzyme through Srb7 and, in turn, enhances gene-specific activation or repression induced by DNA-binding transcription factors (4). Srb7 also interacts with an additional member of the RNAPII holoenzyme, the human homolog of Drosophila TBP-related factor (TRF)-proximal protein (TRFP) (5). TRFP synergistically associates with coactivators, including PC4 (positive coactivator 4) and USA (upstream stimulatory activity) of the RNAPII and SMCC complex, to enhance basal and gene-specific transcription (5,6).
