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p-NF-H Antibody (NAP4): sc-53798

 |  Datasheet

(Based on data analysis)

  • p-NF-H Antibody (NAP4) is a mouse monoclonal IgG1 provided at 50 µl ascites
  • recommended for detection of phosphorylated NF-H of mouse, rat and human origin by WB, IP, IF and IHC(P); also reactive with additional species, including porcine
 

See additional NF Antibodies including NF, NF-H, NF-L and NF-M.

Ordering InformationGene Info
Recommended Support Products:
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WB   IP   IF   IHC(P)   siRNA  
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 Ordering Information
Product NameCatalog #UnitPriceQtyAdd 
p-NF-H Antibody (NAP4) sc-53798 50 µl ascites $279
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
NF-H siRNA (h) sc-42068 10 µM $258
NF-H siRNA (m) sc-42069 10 µM $258
NF-H (h)-PR sc-42068-PR 10 µM, 20 µl $23
NF-H (m)-PR sc-42069-PR 10 µM, 20 µl $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
NF-H shRNA Plasmid (h) sc-42068-SH 20 µg $520
NF-H shRNA Plasmid (m) sc-42069-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
NF-H shRNA (h) Lentiviral Particles sc-42068-V 200 µl $625
NF-H shRNA (m) Lentiviral Particles sc-42069-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
rat brain extract sc-2392 500 µg/200 µl $104
mouse brain extract sc-2253 500 µg/200 µl $104
NF-H (h): 293T Lysate sc-111457 100 µg/200 µl $205
rat cerebellum extract sc-2398 500 µg/200 µl $104
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human NEFH 4744 22q12.2 NM_021076 P12036
 162230
Mouse Nefh 380684 11 A1 P19246
 N/A
 

Neurofilament-H (for neurofilament heavy polypeptide, or NF-H), a member of the intermediate filament family, is a major component of neuronal cytoskeletons. Neurofilaments are dynamic structures; they contain phosphorylation sites for a large number of protein kinases, including protein kinase A, protein kinase C, cyclin-dependent kinase 5, extracellular signal regulated kinase, glycogen synthase kinase-3 and stress-activated protein kinase g. In addition to their role in the control of axon caliber, neurofilaments may affect other cytoskeletal elements, such as microtubules and Actin filaments. Changes in neurofilament phosphorylation or metabolism are frequently observed in neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS), Parkinson's disease and Alzheimer's disease.
p-NF-H Antibody (NAP4) Data
Click on image to enlarge
sc-53798
p-NF-H (NAP4): sc-53798. Western blot analysis of NF-H phosphorylation in non-transfected: sc-117752 (A) and human NF-H transfected: sc-111457 (B) 293T whole cell lysates.
sc-53798
p-NF-H (NAP4): sc-53798. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human cerebellar cortex tissue showing basket cell axon localization.
sc-53798
p-NF-H (NAP4): sc-53798. Western blot analysis of p-NF-H expression in rat spinal cord cell extract.
sc-53798
Western blot analysis of NF-H phosphorylation in non-transfected: sc-117752 (A,D), untreated human NF-H transfected: sc-111457 (B,E) and lambda protein phosphatase (sc-200312A) treated human NF-H transfected: sc-111457 (C,F) 293T whole cell lysates. Antibodies tested include p-NF-H (NAP4): sc-53798 (A,B,C) and NF-H (2D2): sc-20014 (D,E,F).
sc-53798
Western blot analysis of NF-H phosphorylation in untreated (A,C) and lambda protein phosphatase (sc-200312A) treated (B,D) rat brain tissue extracts. Antibodies tested include p-NF-H (NAP4): sc-53798 (A,B) and NF-H (2D2): sc-20014 (C,D).
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