HIF1a Antibody (H1alpha 67): sc-53546

HIF-1α Antibody (H1alpha 67) is a mouse monoclonal IgG2b kappa light chain antibody engineered to recognize the hypoxia-inducible factor 1-alpha (HIF-1α) protein, targeting an epitope within amino acids 432-528 of human HIF-1α. Anti-HIF-1α antibody (H1alpha 67) is essential for researchers studying cellular responses to oxygen deprivation, as HIF-1α plays a critical role in regulating genes involved in glycolysis, erythropoiesis, and angiogenesis, which are vital processes for restoring oxygen homeostasis and maintaining cell growth and viability under hypoxic conditions. HIF-1α, also referred to as HIF1, MOP1, PASD8, and HIF-1alpha, is predominantly located in the cytoplasm under normal oxygen levels, where rapid degradation occurs. However, under hypoxic conditions, HIF-1α stabilizes and translocates to the nucleus, where dimerization with HIF-1β (Arnt1) forms the active HIF-1 complex. This nuclear localization is crucial for HIF-1 binding to hypoxia-responsive elements (HRE) in the DNA, thereby activating transcription of target genes that help cells adapt to low oxygen environments. HIF-1α monoclonal antibody (H1alpha 67) demonstrates broad species reactivity, effectively detecting HIF-1α in mouse, rat, and human samples, as well as in additional species such as porcine, across various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). Available in both conjugated and non-conjugated forms, anti-HIF-1α antibody (H1alpha 67) offers versatility with options like horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, allowing researchers to tailor their experiments to specific detection needs. By facilitating precise localization and quantification of HIF-1α within cellular compartments, HIF-1α (H1alpha 67) antibody is an indispensable tool for advancing our understanding of the molecular mechanisms underlying hypoxia and its impact on various physiological and pathological processes.

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HIF1a Antibody (H1alpha 67) References:

1. Hypoxia-induced angiogenesis during carcinogenesis.  |  Choi, KS., et al. 2003. J Biochem Mol Biol. 36: 120-7. PMID: 12542982
2. Hypoxia-inducible factor (HIF-1)alpha: its protein stability and biological functions.  |  Lee, JW., et al. 2004. Exp Mol Med. 36: 1-12. PMID: 15031665
3. Hypoxia-inducible factor 1 is a basic-helix-loop-helix-PAS heterodimer regulated by cellular O2 tension.  |  Wang, GL., et al. 1995. Proc Natl Acad Sci U S A. 92: 5510-4. PMID: 7539918
4. Endothelial PAS domain protein 1 (EPAS1), a transcription factor selectively expressed in endothelial cells.  |  Tian, H., et al. 1997. Genes Dev. 11: 72-82. PMID: 9000051
5. Molecular characterization of the murine Hif-1 alpha locus.  |  Luo, G., et al. 1997. Gene Expr. 6: 287-99. PMID: 9368100
6. Selection and analysis of a mutant cell line defective in the hypoxia-inducible factor-1 alpha-subunit (HIF-1alpha). Characterization of hif-1alpha-dependent and -independent hypoxia-inducible gene expression.  |  Wood, SM., et al. 1998. J Biol Chem. 273: 8360-8. PMID: 9525945
7. Role of HIF-1alpha in hypoxia-mediated apoptosis, cell proliferation and tumour angiogenesis.  |  Carmeliet, P., et al. 1998. Nature. 394: 485-90. PMID: 9697772
8. Molecular characterization and chromosomal localization of a third alpha-class hypoxia inducible factor subunit, HIF3alpha.  |  Gu, YZ., et al. 1998. Gene Expr. 7: 205-13. PMID: 9840812

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HIF1a Antibody (H1alpha 67) has 195 citations in a variety of scientific publications.

Primary antibodies like Anti-HIF1a Antibody (H1alpha 67) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HIF1a Antibody (H1alpha 67) Specifications

Alternate Product Name: HIF-1α

Product Clone Name: H1alpha 67

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 432-528

Epitope Target: HIF-1α

Epitope Species: human

Product Reactivity/Detection: HIF-1α

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 195 citations in various scientific publications

Available Conjugates/Forms for HIF1a Antibody (H1alpha 67): AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790