p-PACT Background Information Interferon-inducible double stranded RNA-dependent protein kinase activator, also designated PKR-associated protein X (RAX) or PACT, acts as a protein activator of PKR. Following stress such as serum starvation or peroxide or arsenite treatment, PACT associates with and activates PKR, resulting in eIF2å activation (phosphorylation), consequent translation inhibition and cell death via apoptosis. PACT can interact with double stranded RNA (dsRNA), but the eIF2å activation occurs only in absence of dsRNA. The presence of certain growth factors may suppress the pro-apoptotic function of PACT. In both human and mouse cells, PACT is phosphorylated on Serine 18, and the non-phosphorylated form appears to be unable to activate PKR following stress. PACT can form a heterodimer as it interacts with eIF2å through its DRBM domain.
p-PACT (HL1921)
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p-PACT (HL1921): sc-53524. Western blot analysis of phosphorylated PACT expression in immunoprecipitated PACT protein (A) and immunoprecipitated PACT protein dephosphorylated with λPPase (B).
