p55 CDC Background Information Cyclins, regulatory subunits which associate with kinases, control many of the important steps in cell cycle progression. The Cdc2 protein kinase (p34Cdc2) exhibits protein kinase activity in vitro and exists in a complex with both cyclin B and a protein homologous to p13suc 1. Cdc2 kinase is the active subunit of the M phase promoting factor (MPF) and the M phase-specific histone H1 kinase. The p34Cdc2/cyclin B complex is required for the G2 to M transition. An additional cell cycle-dependent protein kinase termed p55 CDC exhibits a high degree of homology with the S. cerevisiae proteins Cdc20 and Cdc4. The p55 CDC transcript is readily detectable in a variety of cultured cell lines in growth phase, but disappears when cell growth is chemically arrested. p55 CDC shows kinase activity towards a-casein and myelin basic protein.
p55 CDC (AR12)
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p55 CDC (AR12): sc-53398. Western blot analysis of p55 CDC expression in HeLa (A), Nocodazole treated HeLa (B) and Ramos (C) whole cell lysates.
p55 CDC (AR12): sc-53398. Western blot analysis of p55 CDC expression in non-transfected: sc-117752 (A) and human p55 CDC transfected: sc-111973 (B) 293T whole cell lysates.
