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Smooth Muscle Actin Antibody (B4): sc-53142

 |  Datasheet

(Based on data analysis)

  • Smooth Muscle Actin Antibody (B4) is a mouse monoclonal IgG1 provided at 200 µg/ml
  • raised against avian gizzard actin
  • recommended for detection of enteric smooth muscle γ-Actin and, to a lesser extent, α-smooth muscle, α-skeletal and α-cardiac (sarcomeric) isoforms of Actin of mouse, rat, human and avian origin by WB, IP, IF and IHC(P); non cross-reactive with cytoplasmic actins
  • See 11 citations for Smooth Muscle Actin Antibody (B4)
 

See additional Actin Antibodies including Actin, alpha-Actin, beta-Actin, gamma-Actin, kappa-Actin, Muscle Actin and Smooth Muscle Actin.

Also see Actin Inhibitors for functional analysis of cellular responses to Actin.


Ordering InformationProduct CitationsGene Info
Recommended Support Products:
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WB   IP   IF   IHC(P)   siRNA  
Set Currency

 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
Smooth Muscle Actin Antibody (B4) sc-53142 200 µg/ml $279
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
C32 Whole Cell Lysate sc-2205 500 µg/200 µl $104
HISM Cell Lysate sc-2229 500 µg/200 µl $104
γ-Actin (h): 293T Lysate sc-116428 100 µg/200 µl $205
A-431 Whole Cell Lysate sc-2201 500 µg/200 µl $104
ACTC1 (m): 293T Lysate sc-126392 100 µg/200 µl $205
ACTG2 (m): 293T Lysate sc-118229 100 µg/200 µl $205
A-10 Cell Lysate sc-3806 500 µg/200 µl $104
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Mouse Acta1 11459 8 E2 P68134
N/A
Mouse Actb 11461 5 G2 P60710
N/A
Mouse Actc1 11464 2 E4 P68033
N/A
Mouse Actg1 11465 11 E2 P63260
N/A
Mouse Actg2 11468 6 C3 P63268
N/A
Mouse Acta2 11475 19 C1 P62737
N/A
 


All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. å-Actin expression is limited to various types of muscle, whereas -∫ and ©-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia.

Smooth Muscle Actin Antibody (B4) Product Citations
See how others have used Smooth Muscle Actin Antibody (B4) and or Smooth Muscle Actin Antibody (B4) conjugates.


11 total citations
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Smooth Muscle Actin Antibody (B4) Data
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Smooth Muscle Actin (B4): sc-53142. Western blot analysis of Smooth Muscle Actin expression in A-10 whole cell lysate.
Smooth Muscle Actin (B4): sc-53142. Western blot analysis of Smooth Muscle Actin expression in PC-12 (A), HeLa (B), NIH/3T3 (C) and KNRK (D) whole cell lysates and mouse heart tissue extract (E).
Smooth Muscle Actin (B4): sc-53142. Western blot analysis of Smooth Muscle Actin expression in HISM (A), A-10 (B), HeLa (C) and C32 (D) whole cell lysates.
Smooth Muscle Actin (B4): sc-53142. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Smooth Muscle Actin (B4): sc-53142. Western blot analysis of ACTG2 expression in non-transfected: sc-117752 (A) and mouse ACTG2 transfected: sc-118229 (B) 293T whole cell lysates.
Smooth Muscle Actin (B4): sc-53142. Western blot analysis of γ-Actin expression in non-transfected 293T: sc-117752 (A), human γ-Actin transfected 293T: sc-116428 (B) and HeLa (C) whole cell lysates.
Smooth Muscle Actin (B4): sc-53142. Western blot analysis of ACTG1 expression in non-transfected 293T: sc-117752 (A), mouse ACTG1 transfected 293T: sc-124922 (B), HeLa (C) and A-431(D) whole cell lysates.
Smooth Muscle Actin (B4): sc-53142. Western blot analysis of ACTC1 expression in non-transfected: sc-117752 (A) and mouse ACTC1 transfected: sc-126392 (B) 293T whole cell lysates.
Smooth Muscle Actin (B4): sc-53142. Immunoperoxidase staining of formalin fixed, paraffin-embedded human smooth muscle tissue showing cytoskeletal staining of smooth muscle cells.
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