Thy-1 Background Information Over 100 cell surface markers have been identified through the use of monoclonal antibodies. Many of these markers have proven useful in identifying specific subpopulations of cells within mixed colonies. Accordingly, these molecules have been assigned a “cluster of differentiation” (CD) designation. One such marker, designated Thy-1 (also referred to as CDw90), is a phosphatidyl-anchored cell surface glycoprotein which, when coexpressed with CD34 on cells from normal human bone marrow, identifies a subpopulation that includes putative hematopoietic, pleuripotent stem cells. Thy-1+ cells from bone marrow have been implicated in syngeneic graft versus host disease and may serve to regulate autoreactivity after bone marrow transplant.
Thy-1 (YBM29/21)
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Thy-1 (YBM29/21): sc-53117. Indirect FCM analysis of mouse peripheral blood leukocytes stained with Thy-1 (YBM29/21), followed by PE-conjugated goat anti-rat IgG: sc-3740. Black line histogram represents the isotype control, normal rat IgG2c.
Thy-1 (YBM29/21): sc-53117. Western blot analysis of Thy-1 expression in CTLL-2 (A) whole cell lysate and mouse brain tissue extract (B) under non-reducing conditions.
