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WASP Antibody (D-1): sc-5300

 |  Datasheet

(Based on data analysis)

  • WASP Antibody (D-1) is a mouse monoclonal IgG1 provided at 200 µg/ml
  • raised against amino acids 1-250 of WASP of human origin
  • recommended for detection of WASP of human origin by WB, IP, IF, IHC(P), FCM and ELISA
  • phycoerythrin conjugate for intracellular FCM, sc-5300 PE, 100 tests
  • Alexa Fluor® 405 (sc-5300 AF405), Alexa Fluor® 488 (sc-5300 AF488) and Alexa Fluor® 647 (sc-5300 AF647) conjugates for FCM or immunofluorescence; 100 µg/2 ml
  • See 17 citations for WASP Antibody (D-1)
 
Full-length WASP protein sequence with immunogen highlighted:
MSGGPMGGRPGGRGAPAVQQNIPSTLLQDHENQRLFEMLGRKCLTLATAVVQLYLALPPGAEHWTKEHCGAVCFVKDNPQKSYFIRLYGLQAGRLLWEQELYSQLVYSTPTPFFHTFAGDDCQAGLNFADEDEAQAFRALVQEKIQKRNQRQSGDRRQLPPPPTPANEERRGGLPPLPLHPGGDQGGPPVGPLSLGLATVDIQNPDITSSRYRGLPAPGPSPADKKRSGKKKISKADIGAPSGFKHVSHGWDPQNGFDVNNLDPDLRSLFSRAGISEAQLTDAETSKLIYDFIEDQGGLEAVRQEMRRQEPLPPPPPPSRGGNQLPRPPIVGGNKGRSGPLPPVPLGIAPPPPTPRGPPPPGRGGPPPPPPPATGRSGPLPPPPPGAGGPPMPPPPPPPPPPPSSGNGPAPPPLPPALVPAGGLAPGGGRGALLDQIRQGIQLNKTPGAPESSALQPPPQSSEGLVGALMHVMQKRSRAIHSSDEGEDQAGDEDEDDEWDD

See additional WASP Antibodies.

Ordering InformationProduct CitationsGene Info
Recommended Support Products:
(click button of application of choice)
WB   IP   IF   IHC(P)   FCM   siRNA  
Set Currency

 Ordering Information
Product NameCatalog #UnitPriceQtyAdd 
WASP Antibody (D-1) sc-5300 200 µg/ml $279
WASP Antibody (D-1) Alexa Fluor® 405 sc-5300 AF405 100 µg/2 ml $314
WASP Antibody (D-1) Alexa Fluor® 488 sc-5300 AF488 100 µg/2 ml $314
WASP Antibody (D-1) Alexa Fluor® 647 sc-5300 AF647 100 µg/2 ml $314
WASP Antibody (D-1) PE sc-5300 PE 100 tests in 2 ml $303
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
WASP siRNA (h) sc-29525 10 µM $258
WASP (h)-PR sc-29525-PR 10 µM, 20 µl $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
WASP shRNA Plasmid (h) sc-29525-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
WASP shRNA (h) Lentiviral Particles sc-29525-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
MOLT-4 Cell Lysate sc-2233 500 µg/200 µl $104
BJAB Whole Cell Lysate sc-2207 500 µg/200 µl $104
Ramos Cell Lysate sc-2216 500 µg/200 µl $104
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human WAS 7454 Xp11.23 NM_000377 P42768
 313900
 

The Wiskott-Aldrich syndrome (WAS) is a disorder that results from a monogenic defect that has been mapped to the short arm of the X chromosome. WAS is characterized by thrombocytopenia, eczema, defects in cell-mediated and humoral immunity and a propensity for lymphoproliferative disease. The gene that is mutated in the syndrome encodes a proline-rich protein of unknown function designated WAS protein (WASP). A clue to WASP function came from the observation that T cells from affected males had an irregular cellular morphology and a disarrayed cytoskeleton suggesting the involvement of WASP in cytoskeletal organization. Close examination of the WASP sequence revealed a putative Cdc42/Rac interacting domain, homologous with those found in PAK65 and ACK. Subsequent investigation has shown WASP to be a true downstream effector of Cdc42.

WASP Antibody (D-1) Product Citations
See how others have used WASP Antibody (D-1) and or WASP Antibody (D-1) conjugates.


17 total citations
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WASP Antibody (D-1) Data
Click on image to enlarge
sc-5300
WASP (D-1): sc-5300. Western blot analysis of WASP expression in MOLT-4 (A), BJAB (B) and Ramos (C) whole cell lysates.
sc-5300
WASP (D-1) PE: sc-5300 PE. Intracellular FCM analysis of fixed and permeabilized BJAB cells. Black line histogram represents the isotype control, normal mouse IgG1: sc-2866.
sc-5300
WASP (D-1) Alexa Fluor 488: sc-5300 AF488. Intracellular FCM analysis of fixed and permeabilized BJAB cells. Black line histogram represents the isotype control, normal mouse IgG1: sc-3890.
sc-5300
WASP (D-1) Alexa Fluor 647: sc-5300 AF647. Intracellular FCM analysis of fixed and permeabilized BJAB cells. Black line histogram represents the isotype control, normal mouse IgG1: sc-24636.
sc-5300
WASP (D-1) Alexa Fluor 488: sc-5300 AF488. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
sc-5300
WASP (D-1): sc-5300. Immunoperoxidase staining of formalin fixed, paraffin-embedded human spleen tissue showing cytoplasmic staining of cells in white pulp.
sc-5300
WASP (D-1): sc-5300. Western blot analysis of WASP expression in non-transfected: sc-117752 (A) and human WASP transfected: sc-170305 (B) 293T whole cell lysates.
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