WASP Antibody (D-1): sc-5300

WASP Antibody (D-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects Wiskott-Aldrich Syndrome Protein (WASP) of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). WASP monoclonal antibody (D-1) is raised against amino acids 1-250 of WASP of human origin. WASP (D-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Wiskott-Aldrich syndrome (WAS) is a genetic disorder linked to a mutation on the short arm of the X chromosome, characterized by symptoms such as thrombocytopenia, eczema, immune deficiencies, and an increased risk of lymphoproliferative diseases. The gene responsible for WAS encodes WASP, a proline-rich protein that plays a crucial role in cytoskeletal organization. WASP is involved in the regulation of actin polymerization, which is essential for various cellular processes, including cell motility, shape, and division. A Cdc42/Rac interacting domain in WASP sequence indicates function as a downstream effector of Cdc42, a small GTPase that orchestrates cytoskeleton reorganization in response to extracellular signals. Understanding WASP′s role in cytoskeletal dynamics is vital, as disruptions in this process can lead to clinical manifestations observed in WAS, highlighting WASP′s importance in maintaining cellular integrity and immune function.

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WASP Antibody (D-1) References:

1. Wiskott-Aldrich syndrome protein and platelets.  |  Oda, A. and Ochs, HD. 2000. Immunol Rev. 178: 111-7. PMID: 11213795
2. Impaired NK-cell migration in WAS/XLT patients: role of Cdc42/WASp pathway in the control of chemokine-induced beta2 integrin high-affinity state.  |  Stabile, H., et al. 2010. Blood. 115: 2818-26. PMID: 20130240
3. Activation of compensatory pathways via Rac2 in the absence of the Cdc42 effector Wiskott-Aldrich syndrome protein in Dendritic cells.  |  Baptista, MAP. and Westerberg, LS. 2019. Small GTPases. 10: 81-88. PMID: 28129089
4. Gene therapy for Wiskott-Aldrich syndrome in a severely affected adult.  |  Morris, EC., et al. 2017. Blood. 130: 1327-1335. PMID: 28716862
5. Wiskott-Aldrich syndrome protein (WASP) is a tumor suppressor in T cell lymphoma.  |  Menotti, M., et al. 2019. Nat Med. 25: 130-140. PMID: 30510251
6. Wiskott-Aldrich syndrome protein forms nuclear condensates and regulates alternative splicing.  |  Yuan, B., et al. 2022. Nat Commun. 13: 3646. PMID: 35752626
7. Wiskott-Aldrich syndrome protein interacts and inhibits diacylglycerol kinase alpha promoting IL-2 induction.  |  Velnati, S., et al. 2023. Front Immunol. 14: 1043603. PMID: 37138877
8. The Wiskott-Aldrich syndrome protein is required for positive selection during T-cell lineage differentiation.  |  Pille, M., et al. 2023. Front Immunol. 14: 1188099. PMID: 37350958
9. A first-in-class Wiskott-Aldrich syndrome protein activator with antitumor activity in hematologic cancers.  |  Spriano, F., et al. 2024. Haematologica. 109: 3602-3614. PMID: 38899342
10. Characterization of the Wiskott-Aldrich syndrome protein and its role in the disease.  |  Nonoyama, S. and Ochs, HD. 1998. Curr Opin Immunol. 10: 407-12. PMID: 9722916

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-WASP Antibody (D-1) has 35 citations in a variety of scientific publications.

Primary antibodies like Anti-WASP Antibody (D-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

WASP Antibody (D-1) Specifications

Alternate Product Name: Wiskott-Aldrich Syndrome Protein

Product Clone Name: D-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-250

Epitope Target: WASP

Epitope Species: human

Product Reactivity/Detection: WASP

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 35 citations in various scientific publications

Available Conjugates/Forms for WASP Antibody (D-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred WASP antibody is WASP Antibody (B-9): sc-13139.