santa cruz biotechnology, inc.
SCBT Logo

Welcome!        Items in Cart     Quick Order

NIFK (18E148) Antibody: sc-52904

 |  Datasheet
  • mouse monoclonal IgG1, 100µg/ml
  • raised against amino acids 100-200 of NIFK of human origin
  • recommended for detection of NIFK of human origin by WB, IP, IF and IHC(P)
 
Additional NIFK Antibodies ...
 
Ordering Information
Recommended Support Products:
(click button of application of choice)
WB   IP   IF   IHC(P)   siRNA  
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human MKI67IP 84365 2q14.3 NM_032390 Q9BYG3
n/a
 
Set Currency

 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
NIFK (18E148) sc-52904 100 µg/ml $279
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
NIFK siRNA (h) sc-72013 10 µM $258
NIFK (h)-PR sc-72013-PR 10 µM $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
NIFK shRNA Plasmid (h) sc-72013-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
NIFK shRNA (h) Lentiviral Particles sc-72013-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
HeLa Whole Cell Lysate sc-2200 500 µg/200 µl $104
BJAB nuclear extract sc-2145 1000 µg $143
Ramos nuclear extract sc-2153 1000 µg $143
HeLa nuclear extract sc-2120 1000 µg $143
CCRF-CEM nuclear extract sc-2146 1000 µg $143
BJAB Whole Cell Lysate sc-2207 500 µg/200 µl $104
K-562 nuclear extract sc-2130 1000 µg $143
NIFK (h2): 293T Lysate sc-159682 100µg/200µl $205

NIFK Background Information
The structural proteins for the complex metalloenzyme nitrogenase include NIFK, NIFD and NIFH. These proteins are all necessary for archaeal and bacterial nitrogen fixation. The NIFK gene encodes the b subunit of the nitrogenase molybdenum-iron (MoFe) tetramer. NIFK localizes to the nucleolus where it interactes with the fork-head associated domain of the proliferation marker protein Ki-67 in a mitosis-specific and phosphorylation-dependent manner. NIFK is widely expressed in adult tissues, suggesting other functions in addition to its interaction with Ki-67, which is only expressed in proliferating cells.

NIFK (18E148)
Click on image to enlarge
NIFK (18E148): sc-52904. Western blot analysis of NIFK expression in Raji whole cell lysate (A) and BJAB (B), Ramos (C) and HeLa (D) nuclear extracts.
NIFK (18E148): sc-52904. Western blot analysis of NIFK expression in non-transfected 293T: sc-117752 (A), human NIFK transfected 293T: sc-113984 (B) and BJAB (C) whole cell lysates.
NIFK (18E148): sc-52904. Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing nucleolar staining of urothelial cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
NIFK (18E148): sc-52904. Western blot analysis of NIFK expression in non-transfected 293T: sc-117752 (A), human NIFK transfected 293T: sc-170742 (B) and Ramos (C) whole cell lysates.
NIFK (18E148): sc-52904. Western blot analysis of mouse NIFK expression in HeLa whole cell lysate.
NIFK (18E148): sc-52904. Western blot analysis of NIFK expression in non-transfected: sc-117752 (A) and human NIFK transfected: sc-159682 (B) 293T whole cell lysates.
Download