Chk2 Background Information Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. Chk1 and Chk2 are involved in these processes as regulators of Cdks. Chk1 and Chk2 both function as essential components in the G2 DNA damage checkpoint by phosphorylating Cdc25C in response to DNA damage. Phosphorylation inhibits Cdc25C activity, thereby blocking mitosis. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. It has also been shown that Chk1 can phosphorylate Wee1 in vitro, providing evidence that the hyperphosphorylated form of Wee1, seen in cells delayed by Chk1 overexpression, is due to phosphorylation by Chk1.
Chk2 (A-12) Product Citations
See how others have used Chk2 (A-12): sc-5278 antibody and or Chk2 (A-12) antibody conjugates.
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Chk2 (A-12)
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Chk2 (A-12): sc-5278. Western blot analysis of Chk2 expression in HeLa (A) and HL-60 (B) whole cell lysates.
Chk2 (A-12): sc-5278. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung tumor showing nuclear staining.
Chk2 (A-12) PE: sc-5278 PE. Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal mouse IgG2b: sc-2868.
Chk2 (A-12): sc-5278. Western blot analysis of Chk2 expression in HL-60 (A) and PMA-treated HL-60 (B) whole cell lysates.
Chk2 (A-12): sc-5278. Western blot analysis of Chk2 expression in non-transfected: sc-117752 (A) and mouse Chk2 transfected: sc-119229 (B) 293T whole cell lysates.
