CD63 Antibody (MX-49.129.5): sc-5275

CD63 Antibody (MX-49.129.5) is a mouse monoclonal IgG1 kappa light chain antibody developed against the full-length human CD63 protein. CD63 monoclonal antibody (MX-49.129.5) reliably detects CD63 across multiple species, including mouse, rat, and human, and is recommended for applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). CD63, also known by alternative names such as LAMP-3, Melanoma-associated antigen ME491, and TSPAN30, is a pivotal member of the tetraspanin family of proteins that facilitate critical processes like cell adhesion, migration, and signal transduction through interactions with specific integrins. Functionally, CD63 plays an essential role in phagocytic activities and the fusion of lysosomes with phagosomes, which are vital for intracellular degradation and recycling mechanisms. CD63 is prominently expressed on activated platelets, monocytes, and macrophages, and is also present at lower levels on granulocytes, T cells, and B cells, highlighting its broad involvement in the immune response. The dynamic translocation of CD63 to the plasma membrane upon platelet activation underscores its importance in cellular signaling and immune function. As a member of the TM4 superfamily of leukocyte glycoproteins, CD63 shares structural features with proteins like CD9, CD37, and CD53, all characterized by four transmembrane domains. Notably, deficiencies in CD63 are associated with Hermansky-Pudlak syndrome, emphasizing its crucial role in normal physiological processes. CD63 (MX-49.129.5) antibody is available in both non-conjugated form and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, offering researchers versatile options to suit diverse experimental needs.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CD63 Antibody (MX-49.129.5) References:

1. Role of adaptor complex AP-3 in targeting wild-type and mutated CD63 to lysosomes.  |  Rous, BA., et al. 2002. Mol Biol Cell. 13: 1071-82. PMID: 11907283
2. Potential role for CD63 in CCR5-mediated human immunodeficiency virus type 1 infection of macrophages.  |  von Lindern, JJ., et al. 2003. J Virol. 77: 3624-33. PMID: 12610138
3. The tetraspanin CD63 enhances the internalization of the H,K-ATPase beta-subunit.  |  Duffield, A., et al. 2003. Proc Natl Acad Sci U S A. 100: 15560-5. PMID: 14660791
4. Peptide YY and neuropeptide Y induce villin expression, reduce adhesion, and enhance migration in small intestinal cells through the regulation of CD63, matrix metalloproteinase-3, and Cdc42 activity.  |  Lee, M., et al. 2005. J Biol Chem. 280: 125-36. PMID: 15504731
5. CD63 as an activation-linked T cell costimulatory element.  |  Pfistershammer, K., et al. 2004. J Immunol. 173: 6000-8. PMID: 15528334
6. CD63 modulates spreading and tyrosine phosphorylation of platelets on immobilized fibrinogen.  |  Israels, SJ. and McMillan-Ward, EM. 2005. Thromb Haemost. 93: 311-8. PMID: 15711748
7. The rat mast cell antigen AD1 (homologue to human CD63 or melanoma antigen ME491) is expressed in other cells in culture.  |  Nishikata, H., et al. 1992. J Immunol. 149: 862-70. PMID: 1634775
8. Novel structurally distinct family of leucocyte surface glycoproteins including CD9, CD37, CD53 and CD63.  |  Horejsí, V. and Vlcek, C. 1991. FEBS Lett. 288: 1-4. PMID: 1879540
9. Trafficking and function of the tetraspanin CD63.  |  Pols, MS. and Klumperman, J. 2009. Exp Cell Res. 315: 1584-92. PMID: 18930046
10. CD63/Pltgp40: a platelet activation antigen identical to the stage-specific, melanoma-associated antigen ME491.  |  Azorsa, DO., et al. 1991. Blood. 78: 280-4. PMID: 2070066
11. Development of a CD63 Aptamer for Efficient Cancer Immunochemistry and Immunoaffinity-Based Exosome Isolation.  |  Song, Z., et al. 2020. Molecules. 25: PMID: 33261145
12. CD63 is regulated by iron via the IRE-IRP system and is important for ferritin secretion by extracellular vesicles.  |  Yanatori, I., et al. 2021. Blood. 138: 1490-1503. PMID: 34265052

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD63 Antibody (MX-49.129.5) has 613 citations in a variety of scientific publications.

Primary antibodies like Anti-CD63 Antibody (MX-49.129.5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD63 Antibody (MX-49.129.5) Specifications

Alternate Product Name: CD63

Product Clone Name: MX-49.129.5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: CD63

Epitope Species: human

Product Reactivity/Detection: CD63

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 613 citations in various scientific publications

Available Conjugates/Forms for CD63 Antibody (MX-49.129.5): AC: Agarose, AF405: Alexa Fluor^® 405, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, TRITC: Rhodamine

The preferred CD63 antibody is CD63 Antibody (E-12): sc-365604.